多发性硬化患者IFN-γ及IL-10分泌性T细胞的变化

The Changes of IFN-γ and IL-10 Secreting T Cells in Multiple Sclerosis Patients

目的 通过计数促炎症性细胞因子IFN γ及抗炎症性细胞因子IL 10分泌性T细胞数目 ,观察多发性硬化 (MS)患者此两类细胞因子 (CK)的变化。方法 采用酶联免疫斑点 (Elispot)技术检测MS患者、其他神经疾病 (OND)患者及正常对照组 (NC)外周血 (PB)及脑脊液 (CSF)中MBP及其他抗原反应性IFN γ和IL 10分泌性T细胞数目 ,并对活动期与缓解期MS及甲基强的松龙 (MP)冲击疗法前后MS患者PB中两种CK分泌性T细胞数目进行了比较。结果 活动期MS患者PB及CSF中IFN γ及IL 10分泌性T细胞数目较OND及NC组明显增多 (P <0 0 5) ,CSF中MBP反应性T细胞差异更为显著 (P <0 0 1)。MS患者PB中IL 10分泌性T细胞数在缓解期较活动期明显升高 (P <0 0 5)。MP治疗后IFN γ分泌性T细胞数目明显减少 ,而IL 10分泌细胞数目明显增多 (P <0 0 1)。结论 在MS发病机制中IFN γ起促进作用 ,而IL 10有保护作用 ,是促、抗炎症性CK的比值而非按顺序的表达决定MS的活动性。

Objective To investigate the changes of cytokines(CK) in multiple sclerosis (MS) by counting the numbers of pro inflammatory CK IFN γ and anti inflammatory CK IL 10 secreting T cells. Method By reverse Elispot assay,the numbers of the two CK secreting cells stimulated by different antigen in PB and CSF of MS,and other neurological diseases (OND) as well as normal controls (NC) were detected.We also compared the difference of numbers of two subsets of secreting T cell,between the exacerbation and remission stage,before and after methylprednisolone (MP) therapy. Results MS patients in the exacerbation stage had more IFN γ and IL 10 secreting T cells in both PB and CSF than OND or NC group(P<0 05),especially when stimulated by MBP and in CSF(P<0 01).In MS remission stage more IL 10 secreting T cells were found(P<0 05).After MP therapy the number of IFN γ secreting T cells decreased greatly while IL 10 sec reting T cells increased(P<0 01). Conclusion IFN γ is a disease promoting CK while IL 10 is a protective CK in MS,it′s the ratio between pro and anti inflammatory CK that determine the clinical phenomena.The mechanism of MP therapy may be partly due to the balancing effect on the CK network.