B7-2(CD86)协调刺激因子在急性实验性变态反应性脑脊髓炎(AEAE)发挥主导作用

Costimulatory Molecule B7-2(CD86)Plays a Dominant Role in Acute Experimental Allergic Encephalomyelitis (AEAE)

目的 探讨协调刺激分子B7 1(CD80 )和B7 2 (CD86)在急性EAE发病过程中的作用。方法 观察抗B7 1和B7 2抗体在体外对淋巴细胞抗原特异性增殖反应和细胞因子分泌的抑制作用和在体内对EAE发生过程的影响。结果 抗B7 2抗体抑制PLP136 150抗原引起的特异性细胞增殖和IL 2产生 ,抗B7 2抗体处理过的淋巴母细胞诱导轻症被动EAE ;在主动EAE诱导的早期抗B7 2抗体虽能延缓发病时间但加重病情 ,可能与IL 4分泌不足有关 ;当临床EAE首发症状出现时 ,抗B7 2抗体减轻其临床表现。结论 协调刺激分子B7

Objective To investigate the dominant roles of costimulatory molecules(B7 1, CD80; B7 2,CD86) in AEAE. Methods Antibodies of anti B7 1 and B7 2 were used to check the proliferation and cytokines production of T cells specific to PLP 136 150 in vitro, and the effects on the active AEAE in vivo. Results Anti B7 2 mAb inhibited the proliferation of cells and the production of IL 2,resulted in diminution of clinical illness with in vitro activated PLP specific T cells; delayed but enhanced the clinical manifestation of EAE and lowered the production of IL 4 when administered at the initial stage of EAE;ameliorated the clinical score as EAE first sign seen. Conclusion Costimulatory molecule B7 2 plays an important role in the induction of AEAE.