聚酰胺胺超支化聚合物的细胞毒性及抗菌性能

Cytotoxicity and antibacterial of hyperbranched polyamidoamine compound

超支化聚合物合成方便、成本低廉,具有高度支化的分子结构及大量末端官能团,在杀菌试剂开发方面具有广阔的前景.以乙二胺及丙烯酸甲酯为单体,通过简单反应得到末端为氨基的聚酰胺胺超支化聚合物,并研究了其作为抗菌试剂的生物活性.以Hela细胞为对象,通过噻唑蓝(MTT)法测试了该超支化聚合物的细胞毒性,并选用大肠杆菌和金黄葡萄球菌为对象,对其抑菌活性进行了研究.结果表明,超支化聚酰胺胺在0～20mg/mL质量浓度范围内毒性较小,细胞存活率在50%以上;超支化聚合物对革兰氏阳性菌金黄葡萄球菌的抑制效果较好,在0.05mg/mL质量浓度下即可实现完全抑制;当质量浓度为12.5mg/mL时,对革兰氏阴性菌大肠杆菌可以完全抑制.该类聚酰胺胺超支化聚合物有望开发成为安全高效的水处理杀菌试剂.

Hyperbranched polymer is a kind of macromolecules with simple synthesis,low cost,highly branched structure and abundant end groups,and has the potential for the development of antibacterial agent.Amine-terminating hyperbranched polyamidoamine was synthesized with ethylenediamine and methyl acrylate as monomers,and its biological activities were investigated.The cytotoxicity of hyperbranched polymer was determined with Hela cells through 3-（4,5-Dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bromide（MTT） strategy.Its antimicrobial activity was assayed with Escherichia coli and Staphylococcus aureus as the bacteria.The results show that hyperbranched polyamidoamine exhibits relatively low cytotoxicity in the concentration range from 0 to 20 mg/mL where more than 50% of the cells maintain viable;the synthetic hyperbranched polymer displays very good inhibition on the propagation of Staphylococcus aureus with an effective concentration of 0.05 mg/mL,while that for Escherichia coli is about 12.5 mg/mL.This synthesized hyperbranched polyamidoamine is a good candidate for safe and efficient antibacterial agent in water treatment.