神经节苷脂对急性低氧大鼠脑皮层神经元的保护作用研究

Protective Effects of Gangliosides on Cerebral Neuronal Damage of Rat during Acute Hypoxia.

目的观察外源性神经节苷脂对急性减压低氧大鼠脑神经元的保护作用 ,为临床应用提供依据。方法 1 5只Wistar大鼠随机分为 :低氧组A、低氧组B与常氧组。低氧组A置于海拔 70 0 0m ,持续 5h ,低氧组B在低氧 3d前腹腔注射神经节苷脂 ( 1 0 0mg/kg/d) ,其余与低氧组A相同。电镜观察各组脑皮层神经元的超微结构改变。结果低氧组B动物的脑神经元胞体、细胞骨架和突触结构损伤的程度明显轻于低氧组A。结论神经节苷脂对低氧性脑损伤有一定的保护作用 ,这可能与其减少低氧时胞内Ca2 +蓄积和稳定CaM水平与CaMKinaseⅡ活性变化有关。

Objective To observe the preventive and therapeutic effects of gangliosides on acute altitude hypoxic brain damage. Method Fifteen Wistar rats, weighed 180～220 g, were randomly divided into 3 groups,hypoxia group A,hypoxia group B and normoxia group. The rats in hypoxia group A were exposed to a simulated altitude of 7000 m for 5 hours and the rats in hypoxia group B were exposed to hypoxia after intraperitoneal administration of mixed gangliosides(100 mg/kg/d) for 3 days. Result Gangliosides could ameliorate the severity of damage in neuronal bodies、cellular skeletons and synapses, especially in synapses. Conclusion It is suggested that gangliosides are effective in preventing and treating hypoxic brain damages by decreasing the accumulation of intracellular Ca 2+ ,and stabilizing CaM and CaM PKII activity during acute hypoxia.