丁鱥体内3株可疑致病真菌的分离鉴定与生物学特性初步研究

Isolation, identification and biological characteristics of 3 suspicious pathogenic fungi from Tinca tinca

从乌江网箱养殖的患病丁鱥(Tinca tinca)鳍上分离到3株可疑致病真菌,分别命名为丁鱥鳍1(DGQ1)、丁鱥鳍2(DGQ2)、丁鱥鳍3(DGQ3)。通过形态学特征和分子生物学方法对其鉴定,结果表明,DGQ1、DGQ2、DGQ3分别为多籽毛霉(Mucedoparvisporus)、变孢毛霉(Mucedo alboater Naumov)和产黄青霉(Penicillium chrysogenum)。采用4种培养基及7种不同温度对该3种真菌进行培养,结果显示,3个菌株在查氏琼脂培养基(CA)、查氏琼脂培养基(CYA)、燕麦脂培养基(OA)上生长状况良好,在25%甘油硝酸盐琼脂培养基(G25N)上生长状况较差;25℃是该3个菌株的最适温度,DGQ1、DGQ2在35℃以上几乎不能生长,DGQ3在35℃可以生长,但生长速度比较慢。

Three plants suspicious pathogenic fungi were isolated from fin of cage cultured sicken Tinca tinca from Wujiang River,named DGQ1,DGQ2 and DGQ3 respectively.Identified by means of morphological feature and molecular biology method,DGQ1 was Mucedo parvisporus,DGQ2 was Mucedo alboater Naumov,and DGQ3 was Penicillium chrysogenum respectively.We trained 3 kinds of fungies by using 4 kinds of culture medium and 7 kinds of temperature.The result displayed that 3 kinds of fungies had good growth on Czapek agar（CA）,Czapek yeast autolysate agar（CYA） and oat meal agar（OA）,but had weak growth on 25% glycerol nitrate agar（G25N）.The most suitable temperature for 3 kinds of fungies growth was 25℃.DGQ1 and DGQ2 barely growed at 35℃,DGQ3 could grow at 35℃ with relatively slow growth rate.