根结线虫诱导巨型细胞形成关键启动子的定位

Identification of the special promoter of giant cell formation induced by root-knot nematodes

为了找到根结线虫诱导的烟草根结内特异性强启动的启动子和了解巨型细胞被诱导形成的分子机理,用无启动子的报告基因(GUS)及旁边的Ds转座子,分离烟草根结内巨型细胞特意启动子。将p13DGUTs转烟草叶片,获得了转基因植株。通过根结线虫感染盆载转基因烟草后,分别检测根系、叶的报告基因的表达情况。结果显示,从转基因植株中筛选到只在根结巨型细胞内表达的转基因植株。

In order to find the root-knot specificity promoter of tobacco induced by root-knot nematode and to understand the molecular mechanism of giant cells formation,by using promoterless reporter gene（GUS）,in the next report gene Ds transposon,used for the separation special promoter of tobacco root-knot in giant cell.The p13DGUTs transgenic tobacco leaves,transgenic plants were obtained.The root knot nematode infection in pots containing transgenic tobacco,expression of report gene in root,leaf were detected respectively.The results showed that we screened the special transgenic plant which expressed GUS gene only in giant cells induced by root-knot nematode.