β1肾上腺素能受体基因单核苷酸多态性与中国北方汉族人群静息心率的相关性

Associations between Gl165C and A145G polymorphism of β_1adrenoceptor and resting heart rate inthe Northern Han Chinese

目的探讨β_1肾上腺素能受体（ADRBl）基因单核苷酸多态性位点G1165C、A145G与中国北方汉族人群静息心率的相关性。方法采用心尖部听诊法记录700例健康受试者坐位静息心率。运用TaqMan等位基因分型技术，采用ABl7900基因检测平台，对受试者ADRBl基因A145G和G1165C位点进行检测。分析上述位点与静息心率之间的关系。结果ADRBl基因A145G位点不同基因型之间的静息心率差异有统计学意义（P〈0．01）。对性别、年龄、吸烟、饮酒、收缩压、舒张压、体质指数、空腹血糖、总胆固醇、甘油三酯、高密度脂蛋白胆固醇、低密度脂蛋白胆固醇、血肌酐以及血尿酸进行校正后，多元线性回归分析显示年龄、吸炯、收缩压、血肌酐、甘油三酯、A145G位点与静息心率相关（P〈0．01）。其中，A145G位点的偏回归系数为2．148（P〈0．05）。男性和女性不同基因型携带者之间的静息心率差异均有统计学意义（P均〈0．05）；采用多元线性回归校正可能的影响因素后显示，男性受试者的A145G位点与静息心率相关（P〈0．05），而女性受试者的A145G位点与静息心率无关（P〉0．05）。总体及性别亚组分析均显示G1165C位点不同基因型携带者之间的静息心率差异无统计学意义（P均〉0．05）；采用多元线性回归校正可能的影响因素后，未显示G1165C位点与静息心率相关（P〉0．05）。结论ADRBl基因A145G位点可能与中国北方男性汉族人群的静息心率相关。

Objective To systematically investigate the possible associations between Gl165C and A145G polymorphism of β_1-adrenoeeptor （ADRB1） and resting heart rate （HRrest） in Northern Han Chinese. Methods HRrest of 700 healthy Northern Han Chinese were measured in the sitting position. SNPs were genotyped by the TaqMan assay. Genotypes were differentiated by analyzing the fluorescence levels of PCR products using an ABI Prism 7900HT Sequence Detector. Results HRrest was significantly lower in A145G AA carriers than in AG and GG carriers （ all P 〈 0. 01 ）. Multiple linear regression analysis showed that age, smoking habits, systolic blood pressure, triglyceride, serum creatinine and A145G polymorphism were associated with HRrest（P 〈 0. 01 ）. A145G was significantly related with HRrest independent of other possible confounding variables, and the partial regression coefficient was 2. 148 （ P 〈 0. 05 ）. After adjusting for other confounding factors, significant association between A145G and HRrest was only found in male subjects（ P 〈 0. 05 ） but not in female subjects （ P 〉 0. 05 ）. Conclusion The A145G polymorphism of ADRB1 gene is associated with HRrest in Northern male Han Chinese.