膀胱尿路上皮癌患者血液中微小RNA的表达

Expression of circulating microRNAs in patients with bladder urothelial carcinoma

目的:通过对膀胱肿瘤患者和非肿瘤患者的血液中微小RNA(microRNA)表达谱的检测,寻找特定的异常表达微小RNA,探讨血液中微小RNA作为膀胱尿路上皮癌早期诊断标记物的价值。方法:将确诊为膀胱尿路上皮癌的6例患者纳入此项研究,另外抽取7名非肿瘤患者血液作为对照组。提取血液中的小分子RNA(smallRNA),并通过高通量测序技术获取膀胱尿路上皮癌患者及对照组血液中微小RNA表达谱,然后利用SPSS17.0的t检验对上述高通量结果进行分析,找出实验组与对照组之间存在差异表达的微小RNA。结果:高通量测序筛选出一部分膀胱尿路上皮癌患者与对照组血液中存在差异表达的微小RNA,其中5个微小RNA上调(hsa-miR-378g、hsa-miR-942、hsa-miR-106a-5p、hsa-miR-142-3p和hsa-miR-374a),另外有许多微小RNA下调。结论:在膀胱尿路上皮癌和非肿瘤人群之间,血液中部分微小RNA的表达可能存在差异。

Objective：To search for differentially expressed microRNAs in circulation and to explore their potential application as non-invasive biomarkers for bladder urothelial carcinoma.Methods： Six bladder urothelial carcinoma patients were recruited into this study,and blood from seven non-tumor patients was included as the controls.Total small RNAs were isolated from the blood.By using high-throughput sequencing technologies,we provided microRNA expression profiles of bladder urothelial carcinoma patients and the control group.The data were analyzed using T test of the SPSS17.0 software to study the expression differences of microRNAs between the two groups.Results： The work identified some microRNAs with differential expression in circulation between the bladder urothelial carcinoma patients and the non-tumor patients.Five microRNAs（hsa-miR-378g,hsa-miR-942,hsa-miR-106a-5p,hsa-miR-142-3p and hsa-miR-374a） were identified to be upregulated in the patients,and the expressions of many other microRNAs were significantly downregulated.Conclusion： The study reveals that there is a variance of microRNA expression profile in circulation between bladder urothelial and non neoplastic populations,The results need further study by large samples.