肾组织干细胞对人肾小管上皮细胞损伤修复的作用

Effects of kidney stem cells on injure-recovery of human renal tubular epithelial cells

目的:研究肾组织干细胞(kidney stem cells,KSC)在急性肾小管损伤过程中的作用及其机制。方法:从大鼠肾乳头分离、提取并鉴定KSC,进行传代培养。利用0.1μmol/L抗霉素A处理人肾小管上皮细胞(human kid-ney epithelial cells,HKC)制作细胞损伤模型。将荧光标记的KSC和损伤的HKC进行共培养,观察各时间点HKC的损伤情况以及KSC的作用方式,同时采用骨髓间充质干细胞(bone marrow stem cells,BMSCs)进行比较研究。结果:与BMSCs比较,在共培养体系中,KSC通过共培养体系微孔膜迁移到下室的细胞数较少。相对于空白对照,与KSC或BMSCs共培养的HKC细胞腺嘌呤核苷三磷酸(ATP)含量以及上清液总超氧化物歧化酶(superoxide dis-mutase,SOD)的值较高,丙二醛(malondialdehyde,MDA)、乳酸脱氢酶(lactate dehydrogenase,LDH)值均下降,采用KSC或BMSCs培养上清液干预时这种现象更加明显,且细胞存活率较高、凋亡率较低。KSC和BMSCs对于损伤HKC的修复作用功能相似。结论:KSC对受损的HKC发挥了抗凋亡、减轻氧化应激和促进修复及增殖的作用。

Objective：To investigate the protective effects and the mechanism of kidney stem cells（KSC） on the injured tubular epithelial cells.Methods： KSC were isolated from rat renal papilla.The human kidney epithelial cells（HKC） injury model was induced with 0.1 μmol/L antimycin A for 30 minutes.The injured HKC were co-cultured with KSC or supernatant of cultured KSC.The apoptosis of HKC were detected by flow cytometry.The changes of ATP in the HKC and the level of malondialdehyde（MDA）,superoxide dismutase（SOD） and lactate dehydrogenase（LDH） in the supernatant of cultured HKC were detected after being co-cultured with KSC.Results： The study of the co-culture showed that KSC was less capable to migrate through the micropores of Transwell compared with bone marrow stem cells.However,after being co-cultured with the KSC conditional supernatant,ATP content of injured HKC,total SOD value in the supernatant of injured HKC were increased,and the MDA and LDH in the supernatant of injured HKC decreased.Conclusion： KSC have protected effects and participate in the repair of injured HKC.