Oct4基因在猪孤雌激活和体外受精早期胚胎中的表达特征

Activation of Oct4 between early parthenogenetic and in vitro-fertilized porcine embryos

目的研究植入前胚胎发育重要基因Oct4在猪孤雌和体外受精胚胎中的表达特征。方法收集成熟卵母细胞、孤雌和体外受精2细胞、4细胞、8细胞胚胎和囊胚,做荧光即时定量PCR检测,以体外成熟的猪卵母细胞做对照分析相对表达量。结果孤雌组和体外受精组胚胎在8细胞期Oct4表达量均最高(P<0.05),在孤雌和体外受精组囊胚相对于其他时期Oct4表达量最低(P<0.05)。在同一时期孤雌和体外受精胚胎上Oct4表达并没有差异。结论多能性基因Oct4在卵裂发育时期表达量动态变化,孤雌胚胎在一定程度上可作为体外胚胎基因表达的模型,且不同的胚胎培养条件可能导致基因表达的差异。

Objective To study the changes of expression of Oct4 in parthenogenetic and in vitro-fertilized （IVF） porcine pre-implantation embryos, which are important for embryonic development. Methods The parthenogenetic and IVF porcine embryos were collected at the 2-cell, 4-cell, 8-cell and blastocyst stages. Quantitative real time PCR was ap- plied to assess the relative amount of each transcription at different embryonic cleavage stages. Results The transcription- al level of Oct4 was the highest one at 8-cell stage （ P 〈 0. 05） , but the mRNA levels of OCT4 in both parthenogenetic and IVF blastocysts were significantly lower than those at 2-cell and 4-cell stages （ P 〈 0.05 ） , except for the status between these two types of embryos. Conclusions Transcriptional changes of pluripotency gene Oct4 exist during their different cleavage stages. In some case, the parthenogenetic embryos can be used as a model for identifying the gene expression changes of those in vitro-produced embryos, and different embryo culture conditions may result in different gene expression ways.