E2F1基因敲除小鼠骨髓造血干、祖细胞减少

Decrease of hematopoietic stem cells and progenitor cells in E2F1 gene knockout mice

目的了解转录因子E2F1基因敲除对造血干、祖细胞的影响。方法用不同抗体标记E2F1基因敲除及同窝野生对照小鼠的外周血、脾脏、骨髓等细胞,应用流式细胞仪进行分析,比较两组之间的差异。结果对检测结果进行统计分析发现,与野生型小鼠相比,E2F1基因敲除小鼠的外周血、骨髓细胞均有改变,脾脏细胞未见明显变化,骨髓前体B细胞、髓系祖细胞、造血干细胞等均有显著变化。骨髓造血干、祖细胞减少,G1期造血干细胞减少。结论 E2F1基因敲除可使小鼠造血干、祖细胞减少。

Objective To study the influence of E2F1 gene knockout on hematopoietic stem cells and progenitor cells. Methods Cells from the peripheral blood, spleen and bone marrow of E2FI knockout and wild type mice were stained with antibodies and analyzed by flow cytometry. Results Compared with the wild type mice, significant differences were found in the pre B cells, myeloid progenitor cells and hematopoietic stem cells in the peripheral blood and bone marrow of the E2F1 knockout mice. The number of hematopoietic stem cells and progenitor cells and percentage of G1 phase hematopoietic stem cells were decreased. Conclusion Hematopoietic stem cells and progenitor cells are decreased in E2F1 gene knockout mice.