热量限制对C57小鼠糖脂代谢的影响

Effects of caloric restriction on glucose and lipid metabolism in C57 mice

目的探讨热量限制(caloric restriction,CR)对小鼠体质量、血糖和血清胆固醇的影响。方法雄性C57小鼠30只,随机分为3组:正常对照组、高能量组、低能量组,每组10只,每2周测体质量和血糖,喂养6个月后用ELISA方法检测小鼠血清总胆固醇。结果高能量组小鼠体质量较正常对照组明显上升(P<0.01),低能量组小鼠体质量较正常对照组明显下降(P<0.01);高能量组小鼠血糖较正常对照组明显上升(P<0.01),低能量组小鼠血糖较正常对照组明显下降(P<0.01);高能量组血清总胆固醇较正常对照组明显增高(P<0.01)。结论热量限制能明显降低小鼠体质量和血糖,高能量饮食明显增高小鼠体质量、血糖和血清总胆固醇水平,热量限制能够改善C57小鼠的糖脂代谢。

Objective To investigate the effect of caloric restriction （CR） on body mass, glucose and total cholesterol levels in C57 mice. Methods Thirty 7-week-old male C57 mice were randomly divided into 3 groups： normal control group, high-energy group and low-energy group, each group consisted of 10 mice. Body mass and blood sugar were measured every two weeks, and the serum total cholesterol was measured by ELISA after six months. Results The body mass of the high-energy group was significantly higher than that of the normal control group （ P 〈 0.01 ）. The body mass of low energy group was significantly lower than that of the normal control group （ P 〈 0.01 ）. The blood glucose of high- energy group was significantly higher than that of the normal control group （ P 〈 0. 01 ）. The blood glucose of low energy group was significantly lower than that of the normal control group （ P 〈 0.01 ）. The serum total cholesterol level of the high-energy group was significantly higher than that of the normal control group （ P 〈 0.01 ）. Conclusions Caloric restriction can significantly reduce the body mass and blood glucose in mice, while high-energy diet can significantly increase the body mass, blood glucose and serum total cholesterol levels in mice. Caloric restriction can improve the glucose and lipid metabolism in C57 mice.