低强度脉冲超声促进兔髌骨-髌腱连接点纤维软骨愈合的形态学分析

Histomorphological analyse of accelerating the fibrocartilage layer repair of patella–patellar tendon junction in rabbits by low intensity pulsed ultrasound stimulation

观察低强度脉冲超声对兔髌骨-髌腱连接点愈合的影响。方法:60只成熟雌性新西兰大白兔按同一标准行髌骨部分切除术,术后按治疗方式随机分成对照组和治疗组。对照组术后给予安慰治疗,治疗组术后3 d^6周给予低强度脉冲超声治疗。分别于术后6,12,18周时处死动物并获取髌骨-髌腱复合体标本,标本常规脱钙后做成切片行H&E染色与番红素染色用于组织学观察。利用SANOMicroscopePartner显微图像测量软件进行组织学分析,比较两组纤维软骨层厚度与灰度值差异。结果:术后6,12和18周时,治疗组的纤维软骨层厚度明显大于对照(P<0.01),灰度值明显小于对照(P<0.01)。结论:低强度脉冲超声可以促进兔髌骨-髌腱连接点纤维软骨层的愈合。

Objective： To analyse the effect of low intensity pulsed ultrasound stimulation （LIPUS） on accelerating the fibrocartilage layer repair of patella-patellar tendon junction. Methods： A total of 60 mature female New Zealand white rabbits undergoing standard partial patellectomy were divided into 2 groups randomly. The control group was given comfort threatment and the treatment group was given LIPUS treatment starting from day 3 to the end of week 6 postoperativel）n The scheduled time points of animal euthanization would be at week 6, week 12 and week 18 postoperatively. The patella-patellar tendon （PPT） complex would be harvested and cut intosections after decalcification for H＆E staining, Safranine o/fast green staining. The thickness and gray value offibrocartilage layer were analyzed by SANO Microscope Partner image analyzer. Results： At week 6, week 12 and week 18 postoperatively, the fibrocartilage layer in the treatment group was significantly thicker than that in the control group （P〈0.01）, and the gray value of fibrocartilage layer was significantly smaller than that in the control group （P〈0.01）. Conclusion： LIPUS helps to accelerate the fibrocartilage layer repair of patella-patellar tendon junction in rabbit models.