硬脊膜完整性可影响脑脊液中细胞因子的水平

Dura mater spinalis integrity may influence cytokine levels in the cerebrospinal fluid

背景:脊髓损伤后的病理生理机制非常复杂,人们对此认识还很不全面、深入。目的:观察脊髓损伤动物模型中硬脊膜完整性对脑脊液内细胞因子水平的影响。方法:采用钳夹压迫法建立新西兰大白兔脊髓损伤模型,随机分为无硬脊膜缺损组、硬脊膜缺损组、硬脊膜缺损复合膜修复组、硬脊膜缺损自体筋膜修复组。术后 30 min、1 h、3 h、6 h、12 h、36 h 采用酶联免疫吸附实验方法检测各组脑脊液中细胞因子白细胞介素 6、白细胞介素 10、肿瘤坏死因子α的变化。结果与结论:无硬脊膜缺损组、硬脊膜缺损复合膜修复组和硬脊膜缺损自体筋膜修复组术后 6 h 脑脊液中白细胞介素 6、白细胞介素 10、肿瘤坏死因子α水平均显著低于硬脊膜缺损组(P < 0.05)。其余时间点 4 组间各因子水平差异无显著性意义(P > 0.05)。说明维护脊髓损伤模型中硬脊膜的完整性可影响脑脊液中白细胞介素6、白细胞介素 10、肿瘤坏死因子α水平,抑制炎症反应。

BACKGROUND： Pathophysioiogical mechanisms after spinal cord injury are very complex, so there is no compressive and in-depth understanding on it. OBJECTIVE： To study the effect of dura mater spinalis integrity on cytokine levels in the cerebrospinal fluid of animal models of spinal cord injury. METHODS： The white rabbit models of spinal cord injury were established using clamp compression method, and then the models were randomly divided into four groups： no dura mater spinalis defect group, dura mater spinalis defect group, dura mater spinalis defect composite with membrane repaidng group and dura mater spinalis defect composite with autologous fascia repair group. Enzyme-linked immunosorbent assay was performed to detect the changes of levels of cytokines （interleukin-6, interleukin-10 and tumor necrosis factor a） in the cerebrospinal fluid at 30 minutes, 1, 3, 6, 12 and 36 hours after surgery. RESULTS AND CONCLUSION： The levels of interleukin-6, interleukin-10 and tumor necrosis factor a in the cerebrospina! fluid of the dura mater spinalis defect group, dura mater spinalis defect composite with membrane repairing group and dura mater spinalis defect composite with autologous fascia repair group were significantly lower than those of the no dura mater spinalis defect group at 6 hours after surgery （P 〈 0.05）. There were no significant differences in the levels of interleukin-6, interleukin-10 and tumor necrosis factor a at other time points between groups （P 〉 0.05）. The results indicate that maintaining the integrity of dura mater spinalis of the spinal cord injury model can affect the leve^s of intedeukin-6, intefleukin-10 and tumor necrosis factor a in the cerebrospinal fluid, thus inhibiting the inflammatory response.