复合同种异体成骨细胞的β-磷酸三钙人工骨修复桡骨缺损

Allogeneic osteoblasts compounded with beta-tricalcium phosphate for repair of radial defects

背景:作为骨支架材料,β-磷酸三钙具有良好的生物相容性、骨诱导性及生物力学性能。目的:探讨β-磷酸三钙复合同种异体成骨细胞修复兔桡骨节段性骨缺损的效果。方法:建立45只兔单侧桡骨骨缺损模型,随机分为3组,实验组缺损区植入复合同种异体成骨细胞的β-磷酸三钙,对照组缺损区植入β-磷酸三钙,空白对照组缺损区不植入任何材料。植入后4,8,16周观察新骨生成情况,通过形态学、X射线等观察指标客观评价各组成骨及骨缺损修复能力。结果与结论:随着修复时间的延长,实验组骨缺损逐渐修复,至16周时X射线见支架与宿主骨之间的骨痂已完全骨化,骨缺损完全修复;组织学见缺损区皮质骨连续,髓腔再通,并且不同时间点实验组修复效果明显优于对照组与空白对照组(P<0.01)。表明复合同种异体成骨细胞的β-磷酸三钙人工骨具有良好的成骨能力,有引导组织再生、防止骨不连的作用。

BACKGROUND： As a bone scaffold material, β-tricalcium phosphate has good biocompatibility, osteoinductive, and biomechanical properties. OBJECTIVE： To study the effect of allogeneic osteoblasts compounded with β-tricalcium phosphate in repairing rabbit radial defects. METHODS： A total of 45 rabbit radial defect models were made and divided into three groups in random. Experimental group was repaired with the compound of allogeneic osteoblasts and β-tricalcium phosphate; control group with β-tricalcium phosphate; and blank control group with nothing. The new bone formation of each group was observed and assessed by X-ray and histopathological analysis at weeks 4, 8, 16 after implantation for evaluation of the bone repairing effect. RESULTS AND CONCLUSION： With the repair time, the experimental group appeared to complete bone defect repair gradually. By the end of 16 weeks, the X-ray showed that the bone callus between the scaffold and the host was completely ossified, and bone defects were completely repaired in the experimental group. Histopathological observation showed continuous cortical bone formed in the defect area, and canal recanalization realized in the experimental group. Additionally, the repair effect in the experimental group was better than that in the control and blank control group at different time points （P 0.01）. It is suggested that the allogenic osteoblasts/β-tricalcium phosphate compound has the better effects on guiding bone regeneration and preventing from nonunion.