脂肪干细胞复合明胶海绵修复兔桡骨缺损

Adipose-derived stem cells combined with gelatin sponge for repair of rabbit radial defects

背景:脂肪干细胞是近年来新发现的一种成体干细胞,有很强的多向分化能力,但其在体内成骨分化的能力研究较少。目的:考察脂肪组织来源间充质干细胞复合明胶海绵修复骨缺损的效果。方法:从兔腹股沟脂肪垫分离和培养脂肪干细胞,用含骨形态发生蛋白2的成骨诱导培养液进行培养,然后注入明胶海绵继续培养。制作兔桡骨缺损模型,将脂肪干细胞-明胶海绵复合体植入实验侧兔桡骨缺损区,对照侧植入明胶海绵。于骨缺损修复后第6,12周处死动物,取标本进行X射线、CT扫描、苏木精-伊红染色作放射学和组织学评价。结果与结论:兔桡骨缺损修复后6,12周,植入明胶海绵-脂肪干细胞复合体的实验侧的Lane-SandhuX射线评分和Lane-Sandhu组织学评分均比对照侧显著升高,说明实验侧骨缺损修复更好。结果表明脂肪干细胞-明胶海绵复合体对兔长骨骨缺损修复有促进作用,具有较明显的体内诱导成骨作用。

BACKGROUND： Adipose-derived stem cells discovered recently are a new kind of adult stem cells, and have a strong multi-differentiation capacity. However, there are rare studies concerning in vivo osteogenic capacity of adipose-derived stem cells. OBJECTIVE： To investigate the effect of adipose-derived stem cells combined with gelatin sponge on repairing bone defects. METHODS： Adipose-derived stem cells from rabbit inguinal fat pads were isolated and cultured, and then induced using an osteogenic medium containing bone morphogenetic protein 2 followed by injection of gelatin sponge. Radial defect models of rabbits were prepared. Compound of adipose-derived stem cells and gelatin sponge was implanted into the lesion side, while gelatin sponge alone was implanted into the contralateral side. Rabbits were killed at weeks 6 and 12 after bone defect repair for X-ray examination, CT scan, and hematoxylineosin staining. RESULTS AND CONCLUSION： Lane-Sandhu X-ray and Lane-Sandhu histological scores after compound implantation were significantly higher than those after repair with gelatin sponge alone. It indicates that adipose-derived stem cells combined with gelatin sponge can promote bone defect healing of rabbits, showing an obvious osteogenic capacity in vivo.