氧化海藻酸钠应用于体外循环管路

Oxidized sodium alginate used in an extracorporeal circulation pipe

背景:目前体外循环肝素涂层管路应用较广,但是价格昂贵,限制了其广泛应用。目的:将多醛基海藻酸钠涂层到医用聚氯乙烯体外循环管路,筛选出最佳涂层条件和所制备管路的稳定性及抗凝性能。方法:利用高碘酸钠氧化海藻酸钠,制备高碘酸钠和海藻酸钠摩尔比分别为1∶8、1∶10和1∶12的多醛基氧化海藻酸钠,采用化学结合的方法将不同氧化度海藻酸钠固定到医用聚氯乙烯体外循环管路表面,筛选出最佳氧化度。以硫酸浓度、聚乙烯亚胺浓度、氧化海藻酸钠浓度、氧化海藻酸钠pH值和温度为因素,采用正交实验筛选最佳涂层条件,评价所制备管路氧化海藻酸钠固定的稳定性及抗凝血性能,并与空白对照组和肝素涂层组进行比较和分析。结果与结论:氧化海藻酸钠最佳氧化度为高碘酸钠和海藻酸钠摩尔比为1∶10。筛选出的最佳涂层条件为50%浓硫酸、0.05%聚乙烯亚胺、反应溶液pH值为3.5、反应温度为40℃和氧化海藻酸钠质量浓度为2g/L。氧化海藻酸钠涂层管路组脱落率与肝素涂层组有相似的结果,抗凝血性能稍劣于肝素涂层组(P<0.05),但显著优于空白对照组。表明氧化海藻酸钠涂层体外循环管路具有良好的抗凝血性能和较好的稳定性。

BACKGROUND： At present, a heparin-coated extracorporeal circulation pipe is used widely, but the price is expensive and limits its wide application. OBJECTIVE： To screen the optimal coating condition of oxidated sodium alginate as a coating material to coat the medical polyvinyl chloride pipe and to evaluate the anticoagulant properties and the stability of the coated pipe. METHODS： Oxidized sodium alginate was prepared with sodium periodate, and the mole ratio of sodium periodate and sodium alginate was 1：8, 1：10 and 1：12, respectively. Oxidized sodium alginate with different degree of oxidation was coated on the surface of medical polyvinyl chloride pipes by chemical methods, then to select the best degree of oxidation. The optimal coating condition was screened through an orthogonal experiment based on concentrations of sulfuric acid, polyethyleneimine and oxidized sodium alginate, and pH value and temperature of oxidized sodium alginate. Additionally, the anticoagulant properties and expulsion rates of the prepared pipes were evaluated in comparison with the blank control group and heparin-coated group. RESULTS AND CONCLUSION： The optimal oxidation degree for oxidized sodium alginate was 1：10 for the mole ratio of sodium periodate and sodium alginate. The best coating conditions were 50% concentrated sulfuric acid, 0.05% polyethyleneimine, 40 ℃ of reaction temperature, 2 g/L oxidized sodium alginate, and pH value=3.5. The oxidized sodium alginate coating group had a similar trend compared with heparin-coated group in the expulsion rate. The anticoagulant properties of oxidized sodium alginate coating group were little weaker than those ofheparin-coated group （P 0.05）, but significantly better than those of the blank control group. The oxidized sodium alginate-coated extracorporeal circulation pipe has a good anticoagulant property and stability.