桂枝挥发油与桂皮醛对病毒性肺炎小鼠死亡保护作用及TLR/IFN信号机制研究

The effects of the volatile oil of Ramulus Cinnamomi and cinnamaldehyde on death protection rate in H1N1 -infected mice and mechanism of TLR/IFN signal pathway

目的:观察桂枝挥发油、桂皮醛的抗甲型流感病毒A/PR/8/34(H1N1)作用,探讨其TLR/IFN信号通路机制。方法:制备流感病毒性肺炎小鼠模型,观察药物对模型小鼠的死亡保护作用;ELISA法测定模型小鼠血清IFN-α、IFN-β水平,Real-Time PCR法测定模型小鼠肺组织TLR7、MyD88、TRAF-6 mRNA的表达量。结果:桂枝挥发油0.348 mg/kg,桂皮醛0.264 mg/kg、0.132mg/kg治疗给药,及桂枝挥发油0.348 mg/kg预防给药,均能明显提高模型小鼠的死亡保护率。桂枝挥发油0.348 mg/kg显著提高模型小鼠血清IFN-α含量及肺组织TLR7 mRNA表达水平,桂皮醛0.264 mg/kg显著升高IFN-β含量。结论:桂枝挥发油、桂皮醛能提高流感病毒性肺炎小鼠的死亡保护率,表现出抗流感病毒作用,抗病毒作用机制与激活TLR7信号通路,促进IFN的分泌有关。

Objective： To study the anti-influenza A virus A /PR /8 /34（H1N1） effect of volatile oil of Ramulus Cinnamomi（VORC） and cinnamaldehyde in vivo,and reveal its mechanism of TLR / IFN signaling pathway.Methods： Mice were infected with H1N1 virus,and the death protection rate of VORC and cinnamaldehyde were observed.The serum levels of IFN-α,IFN-β in H1N1-infected mice were measured by ELISA.Real-time RT-PCR was used to detect the expressions of TLR7,MyD88 and TRAF-6 mRNA in lung tissue.Results： VORC（0.348 mg / kg） and cinnamaldehyde（0.264,0.132 mg / kg） significantly increased the death protection rate in therapeutic administration way,while VORC（0.348 mg / kg） significantly increased the death protection rate in preventive administration way.VORC（0.348 mg / kg） and cinnamaldehyde（0.264 mg / kg） obviously increased the serum levels of IFN-α and IFN-β separately.VORC（0.348 mg / kg） obviously increased the expression of TLR7 mRNA.Conclusion： VORC and cinnamaldehyde could significantly increase the death protection rate in H1N1-infected mice by therapeutic way.The anti-influenza A virus mechanism was related to activating TLR7 signaling pathway and regulating the levels of IFN-α,IFN-β.