丁香酚体外抗阿萨希毛孢子菌作用研究

The antifungal effect of eugenol on trichosporon asahii in vitro

目的：评价丁香酚对阿萨希毛孢子菌的体外抑菌效果,初步探讨其可能的作用机制。方法：参照美国临床实验室标准研究所2008年版酵母菌药敏实验M27-A3方案,测定丁香酚对13株阿萨希毛孢子菌（Trichosporon asahii,T.asahii）的最低抑菌浓度（MIC）及最小杀菌浓度（MFC）;应用紫外分光光度计绘制不同浓度丁香酚作用下阿萨希毛孢子菌的生长曲线;扫描电镜下观察丁香酚对菌体形态学的影响。结果：丁香酚对13株受试菌株的MIC为99.75~399.00μg/ml,MFC为199.50~798.00μg/ml;与空白对照相比,丁香酚作用后的阿萨希毛孢子菌的生长明显受抑制,丁香酚抑菌作用特性表现为较强的浓度依赖性;扫描电镜下可见丁香酚作用后阿萨希毛孢子菌菌体出现肿胀膨大、干瘪变形,部分菌丝胞壁破坏,随着丁香酚药液浓度的增加,可见整个菌丝完全被破坏,胞质外溢。结论：丁香酚对阿萨希毛孢子菌具有较强的抑菌和杀菌作用,可使其形态和超微结构发生明显变化,其作用机制可能为对细胞壁及细胞膜的直接破坏。

Objective： The clearly and evaluation of Chinese medicine monomer-Eugenol on Trichosporon asahii in vitro inhibitory effect.To explore its possible mechanism of action.Methods： Acording to the reference method of National Committee for Clinical laboratory Standards for broth dilution antifungal suscep tibihty testing of yeasts of approved standard-third edition in 2008,assay the minimal inhibitory concentration（MIC） and the minimal fungicidal concentration（MFC） of Eugenol,Trichosporon asahii growth curve of the effect of different concentrations of Eugenol was drown by UV spectrophotometer.Eugenol on cell morphology was observed with scanning electron microscope（SEM）.Results： The MIC of Eugenol against 13 Test strains was 99.75 ~ 399.00 ug / ml,MFC was 199.50 ~ 798.00 ug / ml;Compared with the blank control,Trichosporon asahii,s growth markedly inhibited.The antibacterial activity of Eugenol shows strong concentration dependent and slight time dependent.After exposing to Eugenol,cell swelling swollen,shriveled deformation observed under SEM.Part of the mycelium cell wall damage.With the increase of concentration of Eugenol liquid,visible throughout the mycelium completely destroye,cytoplasm spillover.Conclusion： Chinese medicine monomer-Eugenol has a strong antifungal effect of Trichosporon asahii,make it to morphology and ultrastructure change significantly.