大鼠腹腔注射复方丹参注射液后血清中丹参素的测定

Determination of danshensu in rat serum after intraperitoneal injection of compound Danshen solution

目的:研究大鼠腹腔注射复方丹参注射液后丹参素在血液中的动力学。方法:采用液液萃取法对血清样品进行预处理,以对羟基苯甲酸为内标,甲醇-1%冰醋酸(8∶92)为流动相,用高效液相色谱法于280 nm处检测,保留时间和光谱图进行定性分析。结果:大鼠腹腔注射复方丹参注射液0.7ml(含丹参素3g/ml)后在血清检测到丹参素,用所建立的方法绘制了其经时血药浓度的表格。结论:大鼠腹腔注射血药动力学类型属二室模型一级动力学过程。

Objective： The aim of this research is to study the pharmacokinetics of danshensu after intraperitoneal injection of compound danshen solution in rat serum.Method： Used liquid-liquid extraction to deal with the serum,the conditions for HPLC were as the following： internal standard,4-hydroxy-benzoic acid;the mobil phase,CH 3 OH-1% CH 3 COOH（8∶ 92）;detection wavelength,280nm;qualitative analysis with tR and spectrogram.Result： Danshensu could find in the rat serum after intraperitoneal injection of compound danshen solution 0.7ml（danshensu 3g / ml）,using the established methods to protract the table of serum concentration.Conclusion： The pharmacokinetics of danshensu fitted to two-compartment model,first-order kinetics.