趋化因子受体CXCR3在围绝经期干眼患者中的表达

Expression of chemokine receptor CXCR3 in female patients with dry eye at perimenopause

目的:探讨分析以CXCR3为代表的趋化因子受体家族在围绝经期干眼患者中干眼发生、发展过程中的特征及临床意义。方法:选择围绝经期干眼患者30例,非围绝经期干眼患者30例,用印迹细胞学的方法分别取围绝经期、非围绝经期干眼患者结膜上皮细胞,经流式细胞学分析,比较两组CXCR3阳性细胞表达率的差异。结果:围绝经期干眼组泪膜破裂时间(BUT)、基础泪液分泌(Schirmer I test)值均显著低于非围绝经期干眼组(F=4.076,5.023,P<0.05);围绝经期干眼组CXCR3阳性细胞表达率显著高于非围绝经期的表达率(P<0.01)。CXCR3阳性细胞表达率与泪膜破裂时间、基础泪液分泌值均呈显著的负相关(r=-0.753,-0.684,P<0.05)。结论:可能由于性激素失衡,CXCR3及其配体介导的干眼眼表炎症在围绝经期是高发时期,其表达变化与干眼症临床检查指标密切相关。

AIM： To explore the expression and clinical significance of chemotactic factor receptor-3 （CXCR3） of female patients conjunctival with dry eye at perimenopause. METHODS： Thirty dry eye case （60 eyes） in the patient at perimenopause of the First Affiliated Hospital of Xinjiang Medical University and thirty dry eye cases （60 eyes） in patient at non-perimenopause were selected. The conjunctival epithelial cells at perimenopause and non-perimenopause in dry eye cases were obtained by impression cytology methods, and then immersed into the centrifugal tube with corresponding number respectively and the expression of CXCR3 in conjunctival epithelium of at perimenopause and non-perimenopause in dry eye cases were detected by flow cytometry. RESULTS： The break-up time （BUT） and Schirmer Ⅰ test result of perimenopause was significantly lower than those of non-perimenopause （F=4.076, 5.023; P0.05）. Positive expression rate of CXCR3 at non-perimenopause in dry eye cases was more obvious than that at perimenopause in dry eye syndrome cases （P0.01）. Expression rate of CXCR3 in dry eye cases was negatively correlated with BUT and Schirmer Ⅰ test （r=-0.753, r=-0.684; P0.05）. However, it was non-related with corneal fluorescence staining （P0.05）. CONCLUSION： CXCR3 plays an inflammatory mediators role in dry eye mechanism and its expression level reflects the progress of dry eye at perimenopause.