纳米炭注射液对兔肝癌淋巴引流示踪的实验研究

Experimental study on lymph node tracing with nanocarbon injection in rabbit liver cancer

目的探讨纳米炭注射液用于肝癌淋巴结示踪的价值。方法 2只健康日本大白兔肌内注射VX2细胞株制备荷瘤兔,切除肿瘤后将1mm×1mm×1mm瘤块开腹种植于30只日本大白兔腹腔,制备兔肝癌模型。将30只日本大白兔随机分为对照组、亚甲蓝组与纳米炭组各10只,3组均切除肝癌病灶,清扫局部淋巴结,并于试验结束处死动物,观察肝脏周围淋巴结转移癌数目、示踪剂对淋巴结转移癌的示踪效果。结果对照组、亚甲蓝组和纳米炭组切除淋巴结数目分别为21、14、17个,差异无统计学意义(P>0.05);对照组、亚甲蓝组、纳米炭组对兔肝癌淋巴结示踪的敏感性分别为47.6%、84.2%、87.5%(P<0.05);特异性分别为40.91%、88.89%、86.96%(P<0.01);纳米炭组淋巴结染色时间(100.50±29.92)s较亚甲蓝组(11.20±4.18)s长(P<0.01);纳米炭组淋巴结褪色时间(2.22±0.74)h与亚甲蓝组(1.63±0.54)h比较差异无统计学意义(P>0.05)。结论兔肝癌模型行肝癌病灶切除和淋巴结清扫手术中应用纳米炭注射液对肝癌及淋巴引流有明显示踪作用。

Objective To discuss the value of lymph node tracing with nanocarbon injection to rabbit liver cancer. Methods Rabbit liver cancer models were established by transplanting VX2 ceils with laparotomy in celiac planting method. Thirty Japanese white rabbits were randomly divided into three groups, control group, methylthioninium chloride injection （MC） group and nanocarbon group, with 10 rabbits in each group. The rabbit liver cancer and local lymph nodes were removed in three groups. The number of metastasis lymph nodes and the tracing effect of tracer to lymph node metastasis cancer were observed. Results The numbers of resected lymph nodes were 21, 14 and 17 in control group, MC group and nanocarbon group, with no significant differences （P〉 0.05）. The sensitivities were 47.6%, 84.2% and 87.5%, and specificities were 40.91%, 88.89% and 86.96% respectively in control group, MC group and nanocarbon group, showing significant differences in groups （P〈0.01）. The dyeing time was （100. 50± 29.92） seconds in nanocarbon group, significantly longer than that in MC group （（11.20±4.18） seconds） （P〈0.01）. There was no significant difference in fading time between nanocarbon group （（2. 22 ± 0. 74） hours） and MC group （（1.63±0.54） hours） （P〉0.05）. Conclusion Nanocarbon injection has fine tracer effect on liver cancer and lymph node drainage during resection of liver cancer and lymph node dissection in rabbit liver cancer models.