血清与EDTA-K_2抗凝血浆中降钙素原浓度的比较

A Comparison of Procalcitonin Concentrations in Serum and in EDTA-K_2 Anticoagulated Plasma

目的：分析血浆及血清中降钙素原（procalcitonin，PCT）浓度的差异，探讨是否可用乙二胺四乙酸二钾（EDTA—K2）抗凝血浆代替血清检测PCT含量。方法：应用罗氏2010电化学发光免疫分析仪，分别检测34例血清及EDTA-K2抗凝血浆中的PCT浓度，分析两组数据的相关性，并将检测数据分为3个浓度组（0．05～1μg／L，1～10μg／L，〉10μg／L）进行非参数秩和检验，比较血清及血浆中PCT浓度的差异。结果：血清与EDTA-K2抗凝血浆中PCT浓度具有很好的相关性（r=0．996，P〈0．001），但低、中浓度组的血清及血浆PCT浓度差异具有统计学意义（P〈0．05）。结论：在进一步系统地研究血清与EDTA-K2抗凝血浆中PCT浓度的差异是否具有临床意义之前，暂不建议使用EDTA—K2抗凝血浆检测血液中的PCT含量。

Objective： To Compare procalcitonin （PCT） concentrations in serum and plasma, so as to investigate whether EDTA-K2 plasma could replace serum to be an alternative material for PCT testing. Methods： PCT concentrations in the serum and EDTA-K2 plasma of 34 patients were detected in an immunoanalyzer,and the data were assigned into 3 groups （0. 05 - 1 μg/L, 1 - 10 μg/L, 〉 10 μg/L） for statistic analysis. Results： PCT levels in serum correlated with those in plasma （ r = 0. 996, P 〈0. 001 ） well. However, the differences of PCT concentrations between serum and EDTA-K2 plasma of low-concentration group and medium-concentration group were significant （P 〈 0. 05 ）. Conclusion： The EDTA-K2 plasma is not recommended for PCT testing using electrochemistry luminescence immunoassay before the clinical significance of concentration difference between the serum and plasma being investigated cleadyf