调气活血补肾汤对慢性肾衰竭大鼠肾功能的影响

Impact and Mechanism of Tiaoqihuoxuebushen Decoction on Rats with Chronic Renal Failure

目的:探讨调气活血补肾汤对慢性肾功能衰竭(CRF)大鼠肾功能、肾组织一氧化氮(NO)及脂质过氧化的影响。方法:Wistar大鼠64只随机分为模型组、调气活血补肾汤大剂量治疗组、小剂量治疗组和正常对照组,前3组大鼠麻醉后,用摘除5/6肾脏皮质的方法制备CRF模型;模型制备后,小剂量模型组给予调气活血补肾汤生药13.9 g/kg灌胃,大剂量组给予27.8 g/kg灌胃,模型组给予蒸馏水,各组给药4周、10周分别处死8只大鼠,测定肾功能及NO含量、一氧化氮合酶(NOS)及超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量。结果:大鼠CRF模型成功制备,调气活血补肾汤大剂量组大鼠血清尿素氮(BUN)、肌酐(Cr)明显降低;CRF模型组4周时匀浆NO水平和NOS活性均较对照组明显升高,随时间延长呈下降趋势,大剂量组、小剂量组用药4周NO水平和NOS活性下降,10周时持续降低;且治疗后肾组织SOD活性升高,MDA水平下降,大剂量组均优于小剂量组。结论:调气活血补肾汤可改善肾脏功能,其机制与其调节肾功能NO的代谢平衡、降低自由基损伤有关。

Objective： To investigate the influence of Tiaoqihuoxuebushen decoction on kidney function, kidney NO and lipid peroxidation of Wistar rats with chronic renal failure （CRF）. Methods： Sixty-four Wistar rats were randomly divided into model group （group M）, large-do se treatment group （group L）, small-dose treatment group （group S） and normal control group （group C） with 16 in each. After anesthesia, CRF model were made in the first three groups. Rats in group S were given 13.9 g/ kg of Tiaoqihuoxuebushen decoction via intragastric administration, while those in group L were given 27.8 g / kg of the decoction. Distilled water was given to group M. In 4 w and 10 w after drug administration, 8 rats were executed respectively, and renal function, NO, NOS, SOD and MDA concentrations were measured. Results： CRF model was established successfully, and in group M, NO and NOS activities elevated remarkably than group C in early stage, and decreased along with time. Serum BUN and Cr concentrations in cRF rats were lowered significantly. In groups L and S, NO and NOS activities decreased in 4 w, and kept decreasing in 10 w after treatment, but were still higher than those in groups M and C. Differences between groups were statistically significant （P 〈 0.05 ）. Besides, SOD activities were enhanced significantly after treatment, and decreased in 10 w later. The influences mentioned above were better in group L than those in group S. Conclusion： Tiaoqihuoxuebushen decoction can improve kidney function. Its mechanism is related to lowering free radical injury by enhancing NOS activity and promoting NO composition.