口腔鳞状细胞癌中EGR3基因表达缺失对细胞行为的影响

Loss of expression and mechanism of early growth response gene-3 in human oral squamous carcinoma

目的探讨早期生长反应因子3（EGR3）在口腔鳞状细胞癌（简称鳞癌）组织及肿瘤细胞系中的表达及其对肿瘤细胞生物学行为的影响。方法收集2008年10月—2009年12月北京口腔医院口腔修复科及河南省肿瘤医院肿瘤外科收治的口腔鳞癌患者肿瘤组织样本、配对癌旁样本共20例，对照组口腔黏膜样本7例。明确EGR3在20例口腔鳞癌肿瘤组织、癌旁组织及人口腔鳞癌细胞系KB和Tca-8113的表达情况。构建EGR3全长基因的表达质粒，转染并观察其对口腔鳞癌细胞系Tca-8113细胞增殖、细胞凋亡作用的影响。结果EGR3mRNA在口腔鳞状上皮细胞及癌旁组织相对表达量较高，分别为2．108±0．996和1．721±1．196，在肿瘤组织及口腔鳞癌细胞系中表达量较低，分别为0．007±0．005和0．007±0．001（均P〈0．05）。转染48h后，EGR3转染组肿瘤细胞Tca-8113增殖指数显著低于空质粒对照组（0．35±0．11比0．82±0．21，P〈0．05），Tea-8113中晚期凋亡率（膜联蛋白V／碘化丙啶双阳性细胞群）与空质粒对照组相比差异具有统计学意义（23．12％±6．65％比5．96％±0．98％，P〈0．05）。结论EGR3在口腔鳞癌呈现低表达或表达缺失，EGR3的过表达可显著抑制口腔鳞癌生长、促进肿瘤细胞的凋亡。

Objective To evaluate the expression of early growth response gene-3 （EGR3） in human oral squamous carcinoma tissue, non-adjacent tissue and oral carcinoma cell lines and detect the effects of EGR3 expression on the biological behaviors of oral squamous carcinoma cell lines. Methods From October 2008 to December 2009, the expression of EGR3 was detected in 20 human oral squamous carcinoma tissue, non-tumor adjacent tissue and 2 oral carcinoma cell lines. The total-length plasmid of EGR3 was constructed and transfected into oral squamous carcinoma line Tca-8113 to observe the proliferation and apoptosis. Results There was a high-level expression of EGR3 mRNA in normal oral squamous epithelial and non-tumor adjacent tissues （2. 108 ±0. 996 and 1. 721 ± 1. 196 ）. And a low-level expression or a loss of EGR3 mRNA in human oral squamous carcinoma tissue and oral carcinoma cell lines （0. 007 ±0. 005 and 0. 007 ±0. 001） （both P 〈0. 05）. Tca-8113 transfected by EGR3 plasmid had a high- level expression of EGR3 mRNA capable of inhibiting the proliferation of Tca-8113 and promoting its apoptosis dramatically. The stimulating index in overexpression of EGR3 was lower than control group （0. 35 s0. 11 vs 0. 82 ± 0. 21, P 〈 0. 05 ）. After overexpression of EGR3 in Tca-8113, the frequency of middle-Late stage of apoptosis （ Annexin-V/PI double positive ） was higher than the frequency of vector group （23.12% ±6. 65% vs 5.96% ± 0. 98% , P 〈 0. 05 ）. Conclusion A low-level expression or a loss of EGR3 in human oral squamous carcinoma and an over-expression of EGR3 in human oral squamous carcinoma may inhibit the proliferation and promote apoptosis of oral squamous carcinoma.