结肠癌转移相关基因1蛋白在食管癌组织中的表达及其意义

Significance of MACC1 protein expression in esophageal carcinoma

目的探讨结肠癌转移相关基因1（MACCl）蛋白在食管癌组织中的表达及其意义。方法采用免疫组织化学和Western印迹方法检测了2010至2012年金华市广福医院胸外科手术切除的60例[男38例、女22例，年龄（50±12）岁]食管癌组织及相应癌旁组织中MACC1蛋白的表达情况，并分析其与食管癌临床病理因素之间的关系。结果MACC1蛋白的表达主要位于组织细胞的胞质及胞膜，在食管癌组织中总的阳性表达率明显高于相应癌旁组织[68．3％（41／60）比25．0％（15／60），P〈0．01]；Western印迹结果显示MACC1蛋白在食管癌组织中的表达水平高于癌旁肝组织（0．64±0．05比0．21±0．10，P〈0．05）。MACC1蛋白的表达水平与食管癌的TNM分期和淋巴结转移及分化程度显著相关（均P〈0．05）。结论MACC1的异常表达与食管癌的发生、发展密切相关。

Objective To explore the expression of metastasis-associated colon cancer 1 （MACCI） proteins in esophageal carcinoma and neighboring tissues. Methods The expressions of MACC1 were detected in 60 specimens of esophageal carcinoma and neighboring tissues with immunohistochemistry and Western blotting. All the specimens were selected from 2010--2012 of Guangfu Hospital of Jinhua, 38 males and 22 females, aged （50 ± 12） years. And the correlations of the expressions of MACC1 proteins with the clinicopathologic features of esophageal carcinoma were also analyzed. Results Expression of MACC1 protein was predominantly located in cytoplasm and membrane. The positivity rates of MACC1 protein were 68. 3% （41/60） in esophageal carcinoma tissue and there were significant differences from those in neighboring tissue （25.0 （ 15/60 ）, P 〈 0. 01 ）. Western blotting analysis showed that the expression level of MACC1 protein in esophageal carcinoma was greater than that in corresponding adjacent tissues （ 0. 64 ±0. 05 vs 0. 21±0. 10, P 〈0. 05）. Moreover, the positivity rates and relative expressions of MACC1 showed significant correlations with TNM stage and pathology grade （ all P 〈 0. 05 ）. Conclusion The abnormal expression of MACC1 may be associated with malignant progression of esophageal carcinoma.