摘要
目的:了解食管癌组织中树突状细胞(DCs)在肿瘤浸润性T淋巴细胞激活中的作用。方法:采用免疫组化和RT-PCR的方法,对46例食管癌手术标本中DCs(CD1a)、T细胞亚群(CD3、CD4、CD8、CD25)及共刺激因子B7进行检测。结果:癌组织中的DCs与CD3+、CD4+T细胞有相关性,与CD8+、CD25+T细胞无明显关系,且B7mRNA表达均为阴性。结论:食管癌中DCs不一定能有效地激活T细胞,其中B7基因表达受抑制,可能是不能有效激活T淋巴细胞,产生免疫耐受的原因之一。
Objective To study the role of DCs in activation of infiltrating T lymphocytes in esophageal carcinoma. Methods Forty-six specdimens from patients with esophageal carcinoma were studied for DCs CD10 the subsets of tumor infiltrating T lymphocytes CD3 CD4 CD8 CD25 and costimulatory B7 using monoclonal antibodies by immunohistochemistry staining LSAB method and reverse transcription polymerase chain reaction RT-PCR. Results A significant correlation was found between CD1a+ DCs and CD3+ CD4 T lymphocytes but no remarkable correlation between CD1a+ DCs and CD8+ CD25+ T lymphocytes. No specific B7mRNA expression was found in all specimens. Conclusion DCs may not activate T lymphocytes efficiently. B7 gene expression at low level or deficiency in esophageal carcinoma may be one of the important reasons of failure to activate lymphocyte and induce the immunmosuppression.
出处
《中国肿瘤临床》
CAS
CSCD
北大核心
2000年第9期663-666,共4页
Chinese Journal of Clinical Oncology
基金
河南省自然科学基础研究项目基金!994021700