超抗原活化Th1细胞介导的对肿瘤细胞杀伤效应的研究

Superantigen Activated Th1 Cell Mediated Mechanisms to Kill Tumor Cell Lines

目的 :本文对超抗原葡萄球菌肠毒素B(SEB)活化的Th1细胞介导的对肿瘤细胞杀伤作用进行了研究。方法 :人外周血淋巴细胞与SEB共同培养 ,用流式细胞术测定增殖细胞亚群 ;用酶联双夹心法测定IL 2 ,IL 4水平 ;用K5 6 2 ,HL 6 0肿瘤细胞和自身淋巴细胞作为靶细胞测定效应细胞和细胞因子的杀伤活性。结果 :SEB共同培养 6d的淋巴细胞 ,CD4+T细胞由37.5 %增加到 48.5 % ;CD16 +淋巴细胞由 14.3%增加到 2 7.9% ;CD8+T细胞无明显增加。预先去除CD8+T细胞不影响SEB对CD4+T细胞的活化作用。结论 :SEB活化CD4+T细胞是Th1而不是Th2细胞。它们释放大量的IL 2而不是IL 4。活化的Th1细胞介导了效应细胞和细胞因子对肿瘤细胞的非特异性杀伤效应 。

Objective: We studied the cytotoxic effect of CD4 + Th1 cell mediated against tumor cell lines. Methods: Human peripheral lymphocytes were incubated with Staphylococcal enterotoxin B (SEB) for several days. We measured lymphocyte subsets by flow cytometry, IL 2 and IL 4 lever by ELISA, and cytotoxic activity of NK, cytokines, CTL against K562, HL 60 and isogenous lymphocytes by cell culture. Results: The results showed that CD4 + T cells with SEB stimulation increased from 37.5% to 48.5%. CD16 + lymphocytes increased from 14.3% to 27.9%( P ≤0.05). CD8 + T cells were not proliferative. Activated CD4 + T cells released high level of IL 2 rather than that of IL 4. NK cells and cytokines showed significant effect of killing tumor cell lines, but CTL was not. Conclusion: The results suggested that CD4 + Th1 cells by SEB activating could mediate NK cells and cytokines to kill tumor cell lines but can not regulate anti tumor function of CTL.