rBCG-IL-2对小鼠肿瘤局部和全身免疫功能的影响

Systemic and Local Effects of rBCG-IL-2 on Immunity in Tumor-Bearing Mice

目的 :将rBCG IL 2应用于动物实验 ,观察其对肿瘤生长和免疫功能的影响。方法 :采用基因工程技术构建rBCG IL 2 ,用黑色素瘤细胞株B16接种于小鼠小腿外侧皮下 ,再用rBCG IL 2局部治疗 ,2周后处死小鼠 ,用ELISA法检测脾淋巴细胞培养上清中IL 2 ,IFN γ和GM CSF的含量和肉眼及镜下观察肿瘤生长和局部免疫效应细胞浸润情况。结果 :rBCG IL 2对肿瘤生长的抑制作用明显优于对照组 ,肿瘤细胞周围有较多的巨噬细胞和淋巴细胞浸润 ,肿瘤细胞减少。同时rBCG IL 2还能诱导机体产生IL 2和IFN γ。结论 :rBCG IL 2能通过诱导局部免疫效应细胞浸润和机体细胞因子的产生 ,抑制肿瘤生长 。

Objective: To investigate the effects of rBCG IL 2 on antitumor immunity of the host. Methods: Gene engineering technology was adopted to construct rBCG IL 2. Melanoma cells line B16 was inoculated by hypodermic injection. IL 2, IFN γ and GM CSF in cultivated supernatants of lymphocytes isolated from spleen were determined by ELISA after treatment with rBCG IL 2 two weeks later, the extention of tumor and local immune response cells were observed by pathologic methods. Results: The speed of tumor growth of rBCG IL 2 group was more suppressed dramatically than that of the control group, the number of infiltrating lymphocytes and macrophages increased and the number of tumor cells decreased after treatment with rBCG IL 2. rBCG IL 2 could induced the host to express IL 2 and IFN γ. Conclusion: rBCG IL 2 can suppress even dispel the extension of tumor through inducing the host to express cytokine and enhance the number of immune response cells.