摘要
目的:观察局部冷冻对胶质瘤细胞凋亡的影响,以及不同冻融周期与细胞凋亡之间的关系,进一步探讨冷冻杀伤肿瘤细胞的分子机制。方法:建立胶质瘤动物模型,应用末端标记技术原位检测细胞凋亡, DNA琼脂糖电泳观察 DNA梯形条带。结果:经不同冻融周期冷冻 G422胶质瘤细胞后,在不同时间点,冷冻周围区的肿瘤细胞出现了形态学和 DNA水平上的细胞凋亡变化,其峰值出现在冷冻后 24~ 48 h, 重复冻融后出现更多的凋亡细胞。结论:冷冻治疗可以诱导 G422胶质瘤细胞发生凋亡,细胞凋亡的数目与冻融周期有关。诱导肿瘤细胞凋亡可能是冷冻杀伤肿瘤细胞的重要机制。
Objective: To investigate the effect of cryotherapy on apoptosis in mouse G422 glioma and the relationship between the freeze thaw cycle and apoptosis induction. Methods: Mouse experimental models with glioma was established, TUNEL and DNA gel electrophoresis were applied to detect apoptosis. Results: The results showed that the different freeze thaw cycle could induce apoptosis in G422 glioma cells and the peak of apoptosis was in 24~ 48 h after freezing. Repetition of the freeze thaw cycle induces more apoptotic cells. Conclusions: Cryotherapy could induce apoptosis in mouse G422 glioma, the number of TUNEL positive cells is related to the freeze thaw cycle suggesting that apoptosis induction may be the major mechanism in cryotherapy of glioma with freezing.
出处
《癌症》
SCIE
CAS
CSCD
北大核心
2000年第10期865-867,共3页
Chinese Journal of Cancer
基金
本课题受湖北省教委重点科研基金 !(编号: 96A059)