期刊文献+

局部冷冻对G422胶质瘤细胞凋亡的影响 被引量:5

The effect of legional freezing on apoptosis of G422 glioma
下载PDF
导出
摘要 目的:观察局部冷冻对胶质瘤细胞凋亡的影响,以及不同冻融周期与细胞凋亡之间的关系,进一步探讨冷冻杀伤肿瘤细胞的分子机制。方法:建立胶质瘤动物模型,应用末端标记技术原位检测细胞凋亡, DNA琼脂糖电泳观察 DNA梯形条带。结果:经不同冻融周期冷冻 G422胶质瘤细胞后,在不同时间点,冷冻周围区的肿瘤细胞出现了形态学和 DNA水平上的细胞凋亡变化,其峰值出现在冷冻后 24~ 48 h, 重复冻融后出现更多的凋亡细胞。结论:冷冻治疗可以诱导 G422胶质瘤细胞发生凋亡,细胞凋亡的数目与冻融周期有关。诱导肿瘤细胞凋亡可能是冷冻杀伤肿瘤细胞的重要机制。 Objective: To investigate the effect of cryotherapy on apoptosis in mouse G422 glioma and the relationship between the freeze thaw cycle and apoptosis induction. Methods: Mouse experimental models with glioma was established, TUNEL and DNA gel electrophoresis were applied to detect apoptosis. Results: The results showed that the different freeze thaw cycle could induce apoptosis in G422 glioma cells and the peak of apoptosis was in 24~ 48 h after freezing. Repetition of the freeze thaw cycle induces more apoptotic cells. Conclusions: Cryotherapy could induce apoptosis in mouse G422 glioma, the number of TUNEL positive cells is related to the freeze thaw cycle suggesting that apoptosis induction may be the major mechanism in cryotherapy of glioma with freezing.
出处 《癌症》 SCIE CAS CSCD 北大核心 2000年第10期865-867,共3页 Chinese Journal of Cancer
基金 本课题受湖北省教委重点科研基金 !(编号: 96A059)
关键词 冷冻治疗 胶质瘤 细胞凋亡 DNA 冻融周期 Cryotherapy Glioma Apoptosis
  • 相关文献

参考文献2

  • 1Xu R X,Neurosurgery,1998年,43卷,1期,107页
  • 2陈炳桓,北京医学,1981年,3卷,1期,15页

同被引文献50

  • 1王辉,黄宽明,李新建,秦军,涂汉军,王伦长.冷冻切除联合化疗治疗脑胶质瘤的临床研究[J].肿瘤防治杂志,2005,12(3):237-238. 被引量:1
  • 2任少平,曾惠英,曾显斌,王秋勇,徐朝斌,兰宁.氩氦刀治疗恶性肿瘤的初步临床应用(附15例报告)[J].肿瘤防治研究,2005,32(3):184-184. 被引量:4
  • 3黄宽明,李新建,涂汉军,秦军,张力,付锐,王伦长.冷冻联合rhTNF-α治疗C6大鼠脑胶质瘤的实验研究[J].实用肿瘤杂志,2005,20(4):321-323. 被引量:6
  • 4彭琼,浦佩玉,刘爱学,蔡辉国,陈佩贞,王春艳.脑肿瘤中P53基因突变与突变型P53蛋白表达的研究[J].中华神经外科杂志,1996,12(2):68-71. 被引量:12
  • 5Jerrel W, Benson. Combined chemotherapy and cryosurgery for oral cancer [J]. Am J Surg, 1975, 130:596- 600.
  • 6Fujimura K.Experimental studies on cryochemotherapy [J].Nippon Sanka Fujinka Gakkai Zasshi, 1982, 34(1):21- 29.
  • 7Lee MW, Park SC, Chae HS, et al. The protective role of HSP90 against 3-hydroxykynurenine-induced neuronal apoptosis [J]. Biochem Biophys Res Commun, 2001, 284(2):261- 267.
  • 8Laios E, Rebeyka IM, Prody CA, et al. Characterization of cold-induced heat shock protein expression in neonatal rat cardiomyocytes [J]. Mol Cell Biochem, 1997, 173(1- 2):153- 159.
  • 9Dasgupta G, Moland J. Geldanamycin prevents nuclear translocation of mutant p53 [J]. Exp Cell Res, 1997, 237(1):29- 37.
  • 10Hermisson M, Strik H, Rieger J, et al. Expression and functional activity of heat shock proteins in human glioblastoma multiforme [J]. Neurology, 2000, 54(6):1357- 1365.

引证文献5

二级引证文献9

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部