p16、细胞周期蛋白D1和pRb在头颈部鳞癌的表达及其意义

Expression of p16, cyclinD1 and pRb in head and neck squamous cell carcinoma

目的：探讨 p16、细胞周期蛋白 D1（ cyclinD1）和 pRb在头颈部鳞癌（ head and neck squamous cell carcinoma, HNSCC）的表达及他们的相互关系和其意义。方法：应用免疫组织化学方法检测 32例原发 HNSCC患者癌组织中 p16、 cyclinD1和 pRb的蛋白表达。结果： p16、 cyclinD1、 pRb的总异常率为 90.6％（ 29/32），其中 p16和 pRb蛋白表达缺失分别为 62.5％（ 20/32）和 34.4％（ 11/32）， cyclinD1过表达为 34.4％（ 11/32）。 p16与 pRb呈负相关性（

Objective: To investigate the expression and its significance of p16, cyclinD1 and pRb in human head and neck squamous cell carcinoma(HNSCC), and the relationships among the three proteins. Method: Immunohistochemistry was applied to detect the expression of p16, cyclinD1 and pRb in 32 cases of primary HNSCC. Results: There were 90.6％ (29/32) of primary HNSCC that contained at least one alteration in the p16/ cyclinD1/pRb pathway. The absence of p16 and pRb expression was identified in 62.5％ (20/32) and 34.4％ (11/32) tumors, respectively. Overexpression of cyclinD1 protein was detected in 34.4％ (11/32) HNSCC. There was an inverse correlation between p16 and pRb expression (P