嫩枝扦插不同处理对“香玲”核桃组织再生能力的影响

Effect of Different Softwood Cutting Treatments on the Tissue Regenerating Ability of Juglans regia ‘Xiangling'

以5a生“香玲”核桃为试材，在全光照间歇自动喷雾、细沙基质条件下，研究了扦插时间、不同外源生长调节剂种类及浓度对“香玲”核桃嫩枝组织再生特性的影响。结果表明：在核桃迅速生长的5～8月份，以7月上旬扦插效果最好；IBA处理较2，4-D处理对核桃嫩枝愈伤组织的产生更为显著，且以IBA浓度900-1200mg／kg时效果最好，其中IBA900、1500mg／kg的处理均能诱导插穗分化出根系；不同浓度的IBA处理对插穗内源激素的含量亦有显著影响。

Taking 5-year-old Juglans regia ＇Xiangling＇ as material,the effect of different hormones and different times on its tissue regenerating ability were studied under the full sunlight and automatic intermittent spraying conditions. The results showed that walnut grew rapidly from May to August,and the cutting effect in early July was the best. IBA treatment was better than 2, 4-D treatment for callus induction. IBA 900- 1 200 mg/kg was the most optimal concentration,what＇s more,IBA 900,1 500 mg/kg can induce to grow roots. Different concentrations of IBA treatments had a significant impact on endogenous plant hormones levels in hardwood.