复合树脂、玻璃离子对人巨噬细胞的增殖活性和功能的影响

Effects of composite resin and glass-ionomer cements on proliferation and functional activity of human macrophages

目的:研究常用复合树脂、玻璃离子对体外培养的人单核-巨噬细胞增殖活性和功能的影响。方法:体外诱导培养人的单核血细胞株THP-1,获得巨噬细胞,MTT法测定2种常用复合树脂材料Filtek Z350(3M)、Filtek P60(3M)和玻璃离子材料的24 h浸渍液对体外培养的人单核-巨噬细胞增殖活性的影响,ELISA试验检测各组材料浸渍液对细胞分泌IL-1细胞因子活性的影响。采用SPSS 17.0软件包对数据进行统计学分析。结果:与对照组相比,2种复合树脂材料浸渍培养液对体外培养的人单核-巨噬细胞的增殖活性有促进作用,与对照组相比差异有显著性(P<0.05);检测各材料对巨噬细胞分泌IL-1的影响,2种复合树脂材料与对照组相比,差异没有显著性(P>0.05);玻璃离子与对照组相比,差异有显著性(P<0.05)。结论:复合树脂对人巨噬细胞的增殖和功能活性有显著影响,可能与复合树脂充填产生牙本质敏感症状有关。玻璃离子对人巨噬细胞的增殖活性无明显影响,但可能会促进炎症发展。

PURPOSE： To study the effects of composite resin and glass-ionomer cements on cell proliferation and function of human macrophages in vitro.METHODS： Macrophages were differentiated from THP-1 cells after treatment with phorbol ester and used as the model of inflammatory cells,which were treated by specimens from glass-ionomer cements（GC）,composite resin Filtek Z350（3M）and Filtek P60（3M） on culture medium for 24 hours.The cell proliferation of the tooth-colored restorative materials on human macrophages in vitro was evaluated by MTT color imetric assay,and determined for IL-1 content in these material specimens by ELISA.All statistical analyses were performed using the SPSS 17.0 software package.RESULTS： Compared with control group,composite resin Filtek Z350（3M） and Filtek P60（3M） significantly enhanced the proliferation of human macrophages（P ＆lt;0.05）,while Glass-ionomer had little effect on the proliferation of human macrophages（P ＆gt;0.05）.Glass-ionomer could promote macrophages to secrete IL-1β and the difference was statistically significant（P＆lt;0.05）.The composite resin could not cause release of IL-1β from macrophages（P＆gt;0.05）.CONCLUSIONS： Composite resin enhances proliferation and function of human macrophages.The effect may be associated with hypersensitivity of dentin.Glass-ionomer cement has little effect on proliferation of macrophages,but may lead to progress of inflammation.