交叉象限与上、下半口龈下刮治对龈沟液IL-27水平的影响

Comparison of the IL-27 level in gingival crevicular fluid of cross-quadrant and the upper and lower half-mouth subgingival scaling

目的:检测慢性牙周炎患者采用不同方法进行牙周基础治疗前、后不同时期龈沟液中IL-27的浓度,探讨IL-27在牙周炎发病过程中所起的作用。方法:选择60例中、重度慢性牙周炎患者,随机分为A组(交叉象限刮治)和B组(上、下半口刮治),每组30例,另选牙周健康者30例为C组(对照组)。A组和B组于基线和治疗后1、2、3周分别取样,记录每例患者的各项牙周临床指标。ELISA法检测龈沟液IL-27的浓度。应用SPSS 17.0软件包对数据进行统计学分析。结果:治疗后第2、3周,A、B 2组的PD、AL、BI、PLI均有明显改善,各分组象限与基线比较IL-27浓度均升高,而A组PD、AL、BI及IL-27浓度的变化较B组显著,差异有统计学意义;A组治疗后3周各分组象限IL-27浓度与C组无显著差异。IL-27浓度与PD、AL、BI、PLI呈负相关。结论:龈沟液IL-27可作为牙周病防治及疗效评估的潜在靶分子,交叉象限与上、下半口龈下刮治相比,短期效果更显著。

PURPOSE： To investigate the changes of IL-27 in gingival crevicular fluid（GCF） before and after treatment with different methods for chronic periodontitis.METHODS： Sixty patients with moderate or severe chronic periodontitis were selected as study group to divide into group A（cross-quadrant） and group B（upper and lower half-mouth） randomly.Another 30 healthy people were selected as group C.Gingival crevicular fluid of group A and B were collected at baseline and each week during treatment.At the same time,the clinical parameters including PD,AL,BI and PLI were recorded.And the levels of IL-27 in GCF were measured by ELISA.SPSS 17.0 software package was used for statistical analysis.RESULTS： The levels of IL-27 in GCF 2 and 3 weeks after treatment were higher in study group compared with those at baseline,while PD,AL,BI and PLI showed significant reduction.The changes except PLI in group A was more obvious than group B,which had a significant difference.After three-week treatment,IL-27 in group A had no remarkable difference compared with group C.There were significantly negative correlation between PD,AL,BI,PLI and IL-27.CONCLUSIONS： IL-27 in GCF can be considered as a potential target for prevention and treatment of chronic periodontitis.Compared with the upper and lower half-mouth subgingival scaling,cross-quadrant has more pronounced short-term effect.Supported by Special Research Foundation of Ph.D.Program in University（20112104110013） and Science and Technology Program of Liaoning Province（2012225015）.