EZH2与乳腺癌分子亚型的关系及对临床病理的意义

Expression of EZH2 in breast cancer and its clinicopathological significance

目的:研究EZH2与乳腺癌分子亚型和临床病理参数的关系,探讨EZH2对评价乳腺癌分子亚型预后的价值。方法:回顾分析80例临床病理资料及术后随访资料均完整的乳腺癌患者,通过免疫组织化学Max Vision快捷法,检测ER、PR、HER-2、CK5/6、CK14、EGFR、EZH2蛋白的表达,分析EZH2阳性表达与乳腺癌分子亚型及临床病理参数的关系,观察EZH2对患者预后的影响。结果:80例乳腺癌病例中,Luminal型49例,HER-2(+)型8例,Basal-like型20例,Normal breast-like型3例;各分子亚型只与患者绝经与否具有相关性;Luminal型乳腺癌总生存率最高,Basal-like型最低(P<0.05)。EZH2阳性表达率45.00%,其阳性表达与患者同侧腋窝淋巴结转移与否和肿瘤组织学分级及术后临床分期相关(P<0.05);EZH2在Luminal型、HER-2(+)型、Basal-like型乳腺癌阳性表达率分别为34.60%、50.00%和70.00%,差异有统计学意义(P<0.05)。结论:EZH2阳性表达提示患者预后不良;EZH2有望成为Basal-like型乳腺癌的特异性标记物之一。

Objective： To investigate the expression of EZH2 in breast cancer and its clinicopathologieal significance. Methods： Eighty cases of invasive breast cancer with complete clinlcpathological data and follow- up information were enrolled in the study. The expressions of ER, PR, HER-2, CK5/6, CK14, EGFR and EZH2 proteins were detected by immunohistochemistry （IHC）. The relationship of EZH2 expression with molecular subtypes and prognosis of breast cancer was analyzed. Results： In 80 cases of breast cancer, there were 49 cases of Luminal subtype,8 cases of HER-2 （ ＋ ） subtype ,20 cases of Basal-like subtype and 3 cases of Normal breast-like subtype. The molecular subtypes of breast cancer were associated with menopausal status of patients. Luminal subtype had the highest overall survival rate, while the Basal-like carcinomas were associated with the lowest survival （ P 〈 0. 05 ）. The overall EZH2-positive expression rate was 45.00% and the expression was correlated with axillary lymph node metastasis, histological grading and clinical staging of breast cancer （ P 〈 0.05 ）. The EZH2-positive expression rates in Luminal subtype, HER-2 （ ＋ ） subtype and Basal-like subtype of breast cancers were 34.6% ,50.0% and 70.0% , respectively （P 〈 0.05 ）. Conclusion： Positive expression of EZH2 may indicate a poor prognosis of breast cancer patients and it might be used as a novel therapeutic target for breast cancer of Basal-like subtype.