膀胱肿瘤端粒酶活性及细胞凋亡的研究

Telomerase activity and cellular apoptosis of bladder cancer

目的 探讨端粒酶活性及细胞凋亡与膀胱癌临床生物学行为及预后的关系。 方法 分别采用端粒酶活性试剂盒和末端脱氧核苷酸转移酶介导缺口末端标记 (TUNEL)法对 5 6例膀胱癌标本进行端粒酶活性和细胞凋亡的检测 ,并结合临床资料进行分析。 结果 5 6例膀胱癌标本端粒酶活性阳性率为 89 3 % (5 0 / 5 6 ) ,细胞凋亡指数为 (4 5 2± 14 4) % ;端粒酶活性强度及细胞凋亡指数与年龄、性别、肿瘤大小、数目等无关 (P >0 0 5 ) ;与肿瘤分级、分期及预后有关 (P <0 0 1) ,即端粒酶活性越高或 (和 )细胞凋亡越少 ,则肿瘤“瘤级”、“瘤期”高者预后较差。端粒酶活性强度与细胞凋亡指数呈明显的负相关 (r=- 0 6 9,P <0 0 1)。 结论 膀胱癌端粒酶活性及细胞凋亡与肿瘤分级、分期及预后有关 。

Objective[WT5”BZ] To explore the relationship between clinicobiological acting, telomerase activity and cellular apoptosis of bladder cancer. [WT5”HZ]Methods[WT5”BZ] The intensity of telomerase activity and cellular apoptosis of bladder cancer were detected by telomeric repeat amplification protocol(TRAP) and terminal deoxynucleotidyl transferase mediated dUTP biotin nick end labeling (TUNEL) assay respectively. [WT5”HZ]Results[WT5”BZ] The positive rate of telomerase activity of bladder cancer was 89 29% and the apoptosis index of bladder cancer was (45 16±14 43)%.The intensity of telomerase activity and apoptosis index of bladder cancer were not related to patient′s age, sex, size or number of tumor ( P >0 05),but to the“grade”,“stage”and prognosis of bladder cancer( P <0 01). The higher the intensity of telomerase activity or less cellular apoptosis, the poor prognosis of differenciated or more advanced bladder cancer. The intensity of telomerase activity was negatively correlated with cellular apoptosis of bladder cancer( r =-0 69, P <0 01). [WT5”HZ]Conclusions[WT5”BZ] The intensity of telomerase activity and cellular apoptosis are correlated with the grade,stage and prognosis of bladder cancer.The detection of telomerase activity and cellular apoptosis are helpful in clinical analysis and evaluation of prognosis of bladder cancer. [WT5”HZ]