丙氧鸟苷对K_(562)细胞抑制增殖和诱导分化作用

Growth Inhibition and Erythroid Differentiation Induction of Ganciclovir on K_(562) Cell Lines

目的 研究丙氧鸟苷 (GCV)抑制K562 细胞增殖作用和机制。方法 细胞培养、集落培养、联苯胺染色。结果 0 .6 2 5～ 40 μmol浓度的GCV对K562 细胞有明显的抑制增殖作用 ,作用 4d时的半数抑制量 (IC50 )为 2 .99μmol,作用 7d后克隆形成抑制率达 43% ,联苯胺染色阳性细胞百分率随药物剂量的增加和培养时间的延长而逐渐增加。结论 GCV体外抑制K562

Purpose To evaluate the inhibition of ganciclovir(GCV)on K 562 cell lines proliferation and the mechanism. Methods Cell culture,clone culture and benzidine test was used. Results The proliferation of K 562 cells was inhibited by GCV(0.625-40 μmol).IC 50 at 4 days was 2.99 μmol.The clone formation was inhibited by 43% at 7 days.The percent of benzidine positive cell was increased with exposure to GCV dose or time dependently. Conclusions GCV can inhibit the proliferation of K 562 cell lines and induce erythroid differentiation.