蓝斑参与调节室旁核对心血管活动的作用

Locus coeruleus participates in PVN regulation of cardiovascular activity in anesthetized rats

为探讨化学刺激室旁核对心血管活动的影响及蓝斑在这一效应中的作用 ,给乌拉坦和氯醛糖混合液麻醉的 Wistar系雄性大鼠室旁核微量注射 L-谷氨酸 ,观察了血压、心率和肾交感神经放电活动的变化以及用红藻氨酸损毁蓝斑对刺激室旁核效应的影响。另外 ,为进一步研究室旁核与蓝斑功能联系的机制 ,也观察了蓝斑核区微量注射血管升压素对心血管活动的影响。结果表明 :1室旁核内注射 L-谷氨酸引起血压下降、心率减慢和肾交感神经活动减弱 ;2损毁蓝斑后 ,刺激室旁核引起的心血管效应明显减弱 ;3蓝斑区注射血管升压素导致血压升高、心率加快、肾交感神经放电增加。提示 :1 L-谷氨酸兴奋室旁核抑制心血管活动 ;2室旁核的上述效应可能与交感神经紧张性降低有关 ;3蓝斑参与室旁核对心血管活动的调节过程 ;4血管升压素刺激蓝斑引起心血管活动加强。

To investigate the effect of microinjection of L glutamate ( L Glu) into the paraventricular nucleus of hypothalamus (PVN) on cardiovascular activity and the role of locus coeruleus (LC) in this effect, we observed the blood pressure (BP), heart rate (HR) and renal sympathetic nerve activity (RSNA) after the microinjection, and the effect of LC lesion on stimulating PVN, and the effect of microinjection of vasopressin (VP) into the LC on cardiovascular activity in Wistar rats anesthetized with mixture of urethane and chloralose (i.p). The results showed that:①microinjection of L Glu into the PVN induced a decrease in BP, HR and RSNA;②the response of exciting PVN was attenuated apparently after lesion of the LC with Kainic acid.③microinjection of VP into the LC induced increases in BP, HR and RSNA. These results suggest that:①excitement of PVN by L Glu induces the decrease of cardiovascular activity;②the depressed response to L Glu in the PVN may be related to the decrease in sympathetic nervous tone. ③LC may participate in regulative effect of PVN on cardiovascular activity. ④VP increases cardiovascular activity via stimulation of LC.[