摘要
本文采用反相高效液相色谱法测定人血浆中格列齐特浓度。固定相 :ODS- C18;流动相∶乙腈 -甲醇 -水 (35∶ 2 0∶ 45 )的混合溶液 ,并用磷酸调节 p H至 3.5 ;流速 :1.0 m l· min- 1 ;紫外检测波长 :2 2 8nm ;方法回收率大于 95 % ,日内、日间相对标准偏差小于 8%。血浆的最低检测限为 0 .0 5 μg· m l- 1 ;线性范围 :0 .5～ 12 μg· ml- 1 ,r=0 .9995。
A reverse phase RP HPLC method has been successfully used to determine Gliclazide in plasma.Ananalytical column was packed with ODS C18.The mobile phase was acetonitrile—methanol—water(35∶20∶45)(and phosphate is used to adjust pH=3.5,flow rate of 1.0 ml·min\+\{-1\} UV detection was performed at 228nm.The average recovery was >95%.The respense value was linear between 0.5 μg·ml\+\{-1\}～12 μg·ml\+\{-1\} and r was 0.9995
出处
《海峡药学》
2000年第3期41-42,共2页
Strait Pharmaceutical Journal
