下肢缺血预处理调控未成熟心肌细胞凋亡的研究

The effect of limbs ischemic preconditioning on immature myocardial cell apoptosis in rabbits

目的探讨下肢缺血预处理调控未成熟心肌细胞凋亡和对bcl-2、bax、fas表达的影响。方法采用兔离体心脏Langendorff灌注模型，24只幼兔随机分为三组：对照组（c组，n=8）：仅灌注KH液180rain；缺血／再灌组（I／R组，n=8）：心脏灌注20min后，停灌60min，复灌100min；下肢缺血预处理组（LIP组，n=8），反复3次阻断双下肢血流5min／放开5min，建立Langendorff模型，然后重复I／R组缺血，再灌方法。细胞原位标记与半定量分析细胞凋亡和bcl-2、bax、fas蛋白表达。结果凋亡细胞原位标记与半定量分析：LIP组与I／R组比较，心肌细胞凋亡率明显减少，bcl-2表达明显增多，bax、fas表达明显减少。结论下肢缺血预处理可减少心肌细胞凋亡和调控心肌bcl-2、bax、fas表达。

Objective To investigate the apoptosis of immature myocardial and expression of bcl-2, bax and fas by limbs ischemic preconditioning （LIP）. Methods Isolated working rabbit heart model was used in this study. 24 immature rabbits were randomly divided into 3 groups. Control1 group was only perfused Krebs-Henseleit （KH） 180 minutes. Ischemia/reperfusion group was perfused 20 minutes, and then ischemia 60 minutes followed reperfused 100 minutes. LIP group limbs were repeatly obstructed/relaxed 5 minutes/5 minutes 3 times, and then repeated the method of isehemia/reperfusion of I/R group. The myocardial apotosis and the expression of bcl-2, bax and fas were detected. Results Apoptosis in LIP group was lower than I/R group. The expression of bel-2 in LIP group was higher than I/R group. The expression of bax and fas in LIP group were lower than I/R group. Condu-sion This study demonstrated that LIP can decrease immature myocardial cell apoptosis and regulate the expres- sion of bcl-2, bax and fas.