小鼠心脏移植中转化生长因子β_1表达的实验研究

Expression of TGF-β1 in the grafts of inbred mice heart transplantation

目的研究转化生长因子-β(1transforming growth facter-β1:TGF-β1)在近交系BABL/c小鼠心脏移植中的表达。方法分两组建立近交系BABL/c小鼠颈部心脏移植模型:对照组(n=70)和新赛斯平组(CsA组,n=70),观察移植心脏存活时间,于移植术后第1、3、7、11、14和21天分别取移植心组织各10例,半定量RT-PCR的方法动态检测移植心脏中TGF-β1的表达情况。结果对照组移植心存活时间为(20.3±1.7)d,CsA组移植心存活时间为(32.2±3.4)d,两组间移植心存活时间有显著性差异(P<0.01)。两组中TGF-β1表达的时间变化趋势与移植心存活时间有一致性,但在两组中的表达强度不同,对照组表达较CsA组弱,二者间有显著性差异(P<0.01)。结论 TGF-β1的产生和表达的增加可能与CsA的应用有关,并显著延长了移植物的存活时间。

[ Objective ] To study the expression of TGF-[31 in the grafts of inbred mice with heart transplantation. [Methods] Allografts were divided into 2 groups： control group （n =70）, cyclosporin A-treated group（n =70）. Hearts from inbred BABL/c mice were transplanted into a cervical location in the other recipients and the survival time of the allografts was observed. The local expression of TGF-β1 was detected at day 1, 3, 7, 11, 14, 21 by reverse tran- scription polymerase chain reaction （RT-PCT） respectively. [ Results] The survival time of the allografts was （20.3±1.7） days and （32.2±3.4） days （P 〈0.01）. The levels of the TGF-β1 expression were up-regulated in the group CsA. The up-regulation of TGF-β1 was closely correlated with the survival of the grafts. [Conclusions] The expression and production of TGF-β1 is up-regulated probable cause of administration of cyclosporin A, and favorite to the heart graft survival.