摘要
目的研究转化生长因子-β(1transforming growth facter-β1:TGF-β1)在近交系BABL/c小鼠心脏移植中的表达。方法分两组建立近交系BABL/c小鼠颈部心脏移植模型:对照组(n=70)和新赛斯平组(CsA组,n=70),观察移植心脏存活时间,于移植术后第1、3、7、11、14和21天分别取移植心组织各10例,半定量RT-PCR的方法动态检测移植心脏中TGF-β1的表达情况。结果对照组移植心存活时间为(20.3±1.7)d,CsA组移植心存活时间为(32.2±3.4)d,两组间移植心存活时间有显著性差异(P<0.01)。两组中TGF-β1表达的时间变化趋势与移植心存活时间有一致性,但在两组中的表达强度不同,对照组表达较CsA组弱,二者间有显著性差异(P<0.01)。结论 TGF-β1的产生和表达的增加可能与CsA的应用有关,并显著延长了移植物的存活时间。
[ Objective ] To study the expression of TGF-[31 in the grafts of inbred mice with heart transplantation. [Methods] Allografts were divided into 2 groups: control group (n =70), cyclosporin A-treated group(n =70). Hearts from inbred BABL/c mice were transplanted into a cervical location in the other recipients and the survival time of the allografts was observed. The local expression of TGF-β1 was detected at day 1, 3, 7, 11, 14, 21 by reverse tran- scription polymerase chain reaction (RT-PCT) respectively. [ Results] The survival time of the allografts was (20.3±1.7) days and (32.2±3.4) days (P 〈0.01). The levels of the TGF-β1 expression were up-regulated in the group CsA. The up-regulation of TGF-β1 was closely correlated with the survival of the grafts. [Conclusions] The expression and production of TGF-β1 is up-regulated probable cause of administration of cyclosporin A, and favorite to the heart graft survival.
出处
《中国现代医学杂志》
CAS
CSCD
北大核心
2013年第21期14-17,共4页
China Journal of Modern Medicine
基金
郑州市科技局计划项目[2008]8