邻苯二甲酸二丁酯暴露对大鼠卵巢PPARγ mRNA表达的影响

Effects of dibutyl phthalate on expression of PPARγ mRNA in ovary of rats

目的探讨邻苯二甲酸二丁酯(DBP)对青春期雌性大鼠卵巢PPARγ mRNA表达的影响。方法将32只健康21日龄SPF级SD雌性大鼠按体重随机分为对照(玉米油)组和低(250 mg/kg)、中(500 mg/kg)、高剂量(1 000 mg/kg)DBP染毒组,每组8只。采用灌胃方式进行染毒,染毒容积为5 ml/kg,连续染毒8周。在动情前期处死大鼠,采用RT-PCR法检测大鼠卵巢组织过氧化物酶体增生物激活受体-γ(peroxisome proliferator-activated receptor-γ,PPARγ)mRNA的表达水平。结果与对照组比较,500及1 000 mg/kg DBP染毒组大鼠卵巢PPARγmRNA表达水平升高,差异有统计学意义(P<0.05)。且随着DBP染毒剂量的升高,大鼠卵巢PPARγmRNA的表达水平呈上升趋势。结论 DBP有可能通过激活卵巢PPARγmRNA表达而对雌性大鼠产生生殖毒性。

Objective To study the effect of dibutyl phthalate （DBP） on the expression of PPARγ mRNA in ovary of adolescent rats. Methods 21-day-old Sprague Dawley （SD） female rats were randomly divided into 250,500 and 1 000 mg/kg body weight experimental groups and one control group （corn oil）,the rats were administrated DBP by garage for eight consecutive weeks. The rats were sacrificed in proestrus,the mRNA levels of peroxisome proliferator-activated receptor-γ （PPARγ） of ovaries were detected by real-time fluorescent quantitative polymerase chain reaction （RT-PCR）. Results The expression levels of PPARγ mRNA in ovarian in 500 and 1 000 mg/kg body weight groups were significantly higher than that in the control group （P〈0.05 and P〈0.01）,and the expression level of PPARγ mRNA in 1 000 mg/kg body weight group was higher than those of 250 and 500 mg/kg body weight groups（P〈0.05）. Conclusion DBP exposure may activate the expression of PPAR~/mRNA of ovary, which suggests that DBP exposure may have reproductive toxicity to female rats.