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1998年～2012年鸡源H9N2亚型禽流感病毒NS基因的分子演化规律分析

Molecular and Epidemic Characterization of NS gene of H9N2 Avian Influenza Viruses isolated from Chicken between 1998 and 2012 in China
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摘要 本研究对1998年～2012年分离自我国的32株H9N2亚型禽流感病毒的NS基因进行了克隆和序列测定,并对这些序列进行了系统分析。进化分析表明,在我国流行的H9N2亚型禽流感病毒的NS基因主要属于等位基因A群的BJ/94分支,而属于G1分支的毒株很少被分离到;我国H9N2流行株不断演化出新的亚分支,2000年之前的毒株主要属于Y280亚分支,2007年之后F/98亚分支的毒株逐渐演化为优势谱系。对毒株氨基酸序列的分析表明,部分H9N2亚型禽流感病毒的NS蛋白具有高致病性毒株的分子特征。 Thirty-two nonstructural （NS） genes Of H9N2 subtype avian influenza viruses （AIV）, iso- lated from chicken in China during 1998-2012, were Completely sequenced and phylogenetically analyzed in the research. The results showed that NS genes mainly belonged to BJ/94-1ike lineage Of allele A, and which belonged to Gl-like lineage were rarely isolated. HgN2 subtype epidemic strains constantly evolved into new sub-branches. The NS gene of HgN2 strains before 2000 mainly belonged to Y280-1ike sub-clade, while after 2007 F/98 sub-clade gradually evolved into the dominant lineages. Amino acid sequences analy- sis showed that the NS genes of some HgN2 viruses owned the characteristic animo acids related to high pathogenictity.
作者 郭学金 张毅 王守春 毕玉海 郭妍妍 刘琳琳 尹燕博
机构地区 青岛农业大学动物科技学院 中国动物卫生与流行病学中心 中国科学院微生物研究所 青岛澳兰百特生物工程有限公司
出处 《中国兽医杂志》 CAS 北大核心 2013年第7期3-6,共4页 Chinese Journal of Veterinary Medicine
基金 国家自然科学基金资助项目(31272535)
关键词 H9N2亚型禽流感病毒 NS基因 分子特征 流行规律 H9N2subtype AIV NS gene molecular characteristics epidemiology
分类号 S852.65 [农业科学—基础兽医学]
  • 相关文献

参考文献11

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二级参考文献22

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共引文献28

中国兽医杂志
2013年 第7期

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