发酵过程流加L-谷氨酸提高ε-聚赖氨酸的产量

Enhancement of ε-poly-L-lysine production through L-glutamic acid feeding during fermentation by Streptomyces sp. M-Z18

为了提高Streptomyces sp.M-Z18合成ε-聚赖氨酸(ε-PL)能力,在考察L-谷氨酸添加浓度和添加时机基础上,提出发酵过程中流加L-谷氨酸的策略。结合甘油补料-分批发酵方式,该策略实现174 h内ε-PL发酵产量和产率分别达到31.65 g/L和4.36 g/(L·d),较原发酵工艺分别提高49.2%和43.9%。实验结果表明,发酵过程流加L-谷氨酸是提高ε-PL发酵水平的有效策略之一。

To improve the production of ε-poly-L-lysine （ε-PL） by Streptomyces sp. M-Z18, the strategy of L- glutamic acid feeding during fermentation was developed with the help of optimization of L-glutamic acid supplement concentration and time. Combined with fed-batch fermentation using glycerol as carbon source, the ε-PL production and productivity was reached to 31.65 g/L and 4.36 g/（L . d） respectively in 174 h. Compared with the fermentation without L-glutamic acid addition, the data were increased by 49.2% and 43.9% , respectively. These resuhs demonstrated that the feeding of L-glutamic acid during fermentation was a novel approach for improve ε-PL fermentation level by ε-PL producing strains.