蜂胶乙醇提取液对杏鲍菇及污染菌的抑制作用

The inhibition effect of propolis ethanol extract on Pleurotuseryngii and contaminative microorganisms

以云南西双版纳的粗蜂胶为原料,进行75%、85%、95%的乙醇常温浸提和热浸提,测定蜂胶提取液的黄酮含量,并首次讨论了蜂胶提取液对杏鲍菇及污染菌的抑制作用。结果表明:75%的乙醇热提法提取的黄酮含量最高,为10.51 mg/mL,蜂胶提取液对杏鲍菇及污染菌均有一定的抑制作用,抑制强弱为细菌>杏鲍菇>霉菌。相关性分析表明,杏鲍菇、霉菌和细菌的抑制率与黄酮含量的相关系数均大于0.8,有较强的相关性。在杏鲍菇菌种保藏和子实体的保鲜时,添加适量的蜂胶提取液,可有效抑制细菌的污染。

Using crude propolis from Xishuangbanna of Yunnan as raw materials, different propolis extracts were acquired in 75% , 85% , 95% ethanol at room temperature and by thermal process. The flavonoid contents of propolis extracts were determined. And the inhibition effect of different propolis ethanol extracts on Pleurotuseryngii and contaminative microorganisms was discussed for the first time. The results showed that the highest flavonoids content was 10.51 mg/mL under the conditions of 75% ethanol extraction by thermal process. Propolis extract had inhibition effect on both Pleurotuseryngii and eontaminative microorganisms, the inhibition degree from high to low was as followes ： bacteria 〉 Pleurotuseryngii 〉 Mould. Correlation analysis indicated that all of the correlation coefficients on the inhibitory rate of Pleurotuseryngii, Mould and bacteria with the flavonoids content were above 0.8, which showed a good correlation. Addition of the right amount propolis extract can effectively inhibit bacterial pollution in Pleurotuseryngii species preservation and fruit body preservation.