摘要
建立一种凝胶渗透色谱-高效液相色谱法测定食用植物油中苯并（a）芘含量的方法。样品经凝胶渗透色谱净化处理,采用反相C18色谱柱Phenomenex Luna C18（4.6mm×250mm,5μm）,以乙腈：水=91：9溶液为流动相,流速1mL/min,荧光检测器,激发波长384nm,发射波长406nm,对食用植物油中的苯并（a）芘进行检测。在2.0、10.0、25.0μg/kg添加量范围内,平均回收率为98.7%,相对标准偏差为3.4%（n=6）。方法的检出限为0.3μg/kg,线性范围为0.1~20.0μg/L（R=0.9992）,测定结果与标准方法基本相同。本法快速、简便、准确,可以作为食用植物油中苯并（a）芘的检测方法。
This study developed a reverse phase HPLC method for determing benzo （a） pyrene in edible vegetable oils. After samples were pretreated by gel permeation chromatography, benzo （a） pyrene was separated on a Phenomenex Luna C18 （4.6 mm×250 mm, 5 μm） using a mobile phase composed of acetonitrile and H20 （91：1, v/v） at a flow rate of 1 mL/min and detected using a fluorescence detector set at λex=384 nm, λem=406 nm. At the spike levels of 2.0, 10.0, 25.0 μg/kg, the average recovery for benzo （a） pyrene was 98.7% with the RSD of 3.4% （n=6）, the limit of detection was 0.3 pg/kg, and the linear range was from 0.1-20.0 μg/L （R=0.9992）. The results of benzo （a） pyrene determination in four different edible vegetable oils samples by this method were almost the same as those determined by GB/ T 22509--2008. The method was fast, easy and accurate. Therefore, the method developed can be use to determine benzo （a） pyrene in edible vegetable oils.
出处
《食品科技》
CAS
北大核心
2013年第8期294-297,共4页
Food Science and Technology
基金
浙江省质量技术监督系统科研计划项目(20110236)
