蕨菜乙醇提取物不同萃取部位抗氧化活性测定及其组分HPLC分析

Antioxidant activities and HPLC analysis of different parts partitioned from the ethanol extract of Pteridium aquilinum

目的:对蕨菜乙醇提取物不同萃取部位抗氧化活性测定和组分分析以追踪蕨菜抗氧化活性药效物质基础。方法:乙醚预处理的脱脂蕨粉经75%乙醇提取后以石油醚、氯仿、乙酸乙酯、正丁醇依次萃取,获得石油醚相、氯仿相、乙酸乙酯相、正丁醇相及水相5个部位,通过测定对超氧阴离子、羟自由基的清除能力评价各组分的活性,HPLC法对总提物及5个部位的组分进行对比分析。结果:总提物及五个萃取部位活性明显不同,乙酸乙酯相和氯仿相的抗氧化活性明显高于总提物和正丁醇相,石油醚相和水相抗氧化性较弱。各项的组分存在显著差异。结论:蕨菜乙醇提取物经石油醚、氯仿、乙酸乙酯、正丁醇依次萃取,可实现组分的初步分离。其中黄酮类化合物含量较高的乙酸乙酯萃取部位,与总提物相比,体外抗氧化活性显著提高,且呈明显的量效关系,黄酮是蕨菜主要的抗氧化活性物质之一。研究结果对探讨蕨菜乙醇提取物抗氧化活性物质基础,建立蕨菜质量评价标准、蕨菜资源的深度开发奠定基础。

Objective：To investigate the potential antioxidant constituents of Pteridium aquilinum using bioassayguided method.Methods：75% ethanol extraction from dry P.aquilinum power having been primed by ether was partitioned by petroleum ether, chloroform, ethyl acetate, butanol to afford five fractions.Then the antioxidant activity and chemical constituents of the five extracting parts together with the total ethanol extract were compared by detecting the removing ratio to superoxide anion and hydroxyl radical and HPLC method, respectively. Results： Remarkable variety in both chemical constituents and antioxidant was existed between the extractions. Conclusion.The potential antioxidant components were mainly in ethyl acetate, in which the flavonoids were obviously much more abundant than the other parts. This might provide us new strategy for searching new antioxidant from this flavonoid-rich plant and revealing the possible pharmacodynamic material basis of the antioxidant activity of P.aquilinum.