两歧双歧杆菌F-35对Caco-2细胞中α-葡萄糖苷酶活及葡萄糖转运的影响

Effect of Bifidobacterium bifidum F-35 on α-glucosidase enzyme activity and the transportation of glucose

本文以Caco-2细胞建立的Transwell模型研究了两歧双歧杆菌F-35的发酵上清和细胞内容物对葡萄糖转运和α-葡萄糖苷酶活性的影响及其对α-葡萄糖苷酶(S-I)、钠-葡萄糖共转运体-1(SGLT-1)、葡萄糖转运蛋白-2(GLUT-2)基因表达的影响。结果表明,两歧双歧杆菌F-35的发酵上清和细胞内容物对α-葡萄糖苷酶活性的抑制率分别为13.3%和21.0%,对葡萄糖的转运抑制率分别为15.0%和29.7%;使S-I基因表达水平分别下调3.3倍和3.7倍,SGLT-1基因表达水平分别下调3.5倍和1.6倍,GLUT-2的基因表达水平分别下调4.4倍和1.3倍,与对照组相比均有显著性差异。研究表明,两歧双歧杆菌F-35可通过对α-葡萄糖苷酶活性和葡萄糖转运的抑制及对S-I、SGLT-1和GLUT-2 mRNA表达量的抑制,来延缓餐后碳水化合物水解和影响葡萄糖吸收,具有潜在的降血糖作用。

In this paper, the supernatant and cell free extract of Bifidobacterium bifidum F-35 inhibited the α-glucosidase activity and the glucose transporter by Caco-2 cells and mRNA expressions of α-glucosidase （S-I）, sodium - glucose cotransporter 1 （ SGLT- 1 ）, glucose transporter protein 2 （ GLUT - 2 ） in real - time fluorescence quantitative PCR were studied. The results showed that the α-glucosidase inhibition rate of supernatant and cell free extract was 13.3% and 21.0%,glucose transporter inhibition rate was 15.0% and 29.7%, respectively.S-I gene expression levels was reduced by 3.3 times and 3.7 times by the supernatant and cell free extract of Bifidobacterium bifidum F-35.The SGLT-1 gene expression levels was reduced by 3.5 times and 1.6 times The level of gene expression of glucose transporter protein GLUT-2 was reduced by 4.4 times and 1.3 times respectively in real-time fluorescence quantitative PCR. There was a significant difference compared with the control group. Bifidobacterium bifidum F-35 should delay postprandial carbohydrate hydrolysis and inhibit the glucose absorption by inhibiting α-glucosidase activity and reducing the S-I, SGLT- 1, GLUT-2 mRNA expression levels.Bifidobacterium bifidum F-35 had the potential effect of reducing blood glucose.Further research is needed to evaluate the antidiabetic effect in vivo.