核磁共振法快速检测肝素钠粗品

Fast Determination of Crude Heparin Sodium by NMR Spectroscopy

采用1 H NMR对肝素钠、多硫酸软骨素标准品进行测试,并对谱图进行了归属,建立了核磁共振法快速检测肝素钠粗品的分析方法。结果表明:用500MHz核磁共振波谱仪器进行检测,样品浓度为35mg/0.6mL时,能快速(5min)检测出粗品肝素钠中是否存在杂质多硫酸软骨素,检出限为0.3%。

A method to detect over sulfated chondroitin sulfate（OSCS） contaminant in heparin sodium was established by 1H NMR spectroscopy. 0.3 % OSCS in crude heparin sodium could be detected in 5 minutes. The results showed that, 1H NMR spectroscopy can be used for the quantitative determination of contaminants in heparin.