转pepc基因油菜及其光合生理表现

Photosynthetic Performance of Transgenic Brassica napus with C_4-type pepc Gene

采用下胚轴侵染法和花序浸染法将玉米C4型pepc光合基因导入到甘蓝型油菜中,对转基因植株进行PCR鉴定并测定磷酸烯醇式丙酮酸羧化酶(PEPC)活性及其相关光合生理指标。结果表明,2种转基因方法均能将玉米C4型pepc基因导入油菜中。转pepc基因油菜的磷酸烯醇式丙酮酸羧化酶(PEPC)平均活性比原种提高17倍左右,净光合速率(Pn)比原种提高15.5%,胞间CO2摩尔分数(Ci)比原种降低35%。表明pepc基因在转基因油菜中得到高效表达。

It’s a research hotspot in crop breeding that C4photosynthesis genes are transferred into C3 plants in order to improve the photosynthetic efficiency and crop yields.The methods of hypocotyl infection and inflorescence dipping were used in our research for obtaining transgenic Brassica napus with C4pepc photosynthetic gene from maize.With the untransformed Brassica napus and transgenic Brassica napus as the materials,PCR test,the activity of C4photosynthetic enzymes and photosynthetic physiological indexes were conducted.The results showed that the C4pepc gene from maize can be transfered into Brassica napus by methods of hypocotyl infection and inflorescence dipping,respectively.The average activity of PEPC enzyme in transgenic plants increased by 17times and the photosynthetic rate increased by 15.5%than that in the control.The intercellular CO2molilaty fraction decreased by 35%than the untransformed plants.Based on the above results,it can revealed that the C4 pepc photosynthetic gene in maize were highly expressed in transgenic Brassica napus.This study would provide technical references for Brassica napus breeding with high light efficiency and high grain yield.